Nikolic D M
Br J Biomed Sci. 2014;71(2):73-8. doi: 10.1080/09674845.2014.11669968.
This study aims to determine the origin of Candida contamination of pancreatic tissue cultures, as well as its influence on insulin secretory activity of the pancreatic islets. Pancreatic tissue was obtained after pancreatectomy in patients who had chronic pancreatitis or benign tumours. Islets were isolated under aseptic conditions by a manual method. Microbiological analysis was performed by standard procedures and secretory activity was determined on the first, third and seventh day of cultivation. Insulin stimulation index (SI) on the first day of incubation was 0.665 +/- 0.082 and 0.982 +/- 0.167 for sterile and infected cultures, respectively (expressed as means +/- SE). On the third day of cultivation, the SI for sterile cultures was 0.645 +/- 0.071 while these value were higher in contaminated cultures (1.252 +/- 0.413). On the seventh day, SI was 0.853 +/- 0.032 and 1.239 +/- 0.169 for sterile and infected cultures, respectively (P = 0.05). Analysis of results for the first, third and seventh day of incubation and comparison of both groups showed that SI was 0.721 +/- 0.041 for sterile cultures, while for contaminated cultures it was higher by 37.68% (SI = 1.157 +/- 0.154; P = 0.01). The results show that cell culture contamination originates from an original pancreatic tissue infection, and that Candida can provoke an elevated level of insulin secretion in such patients, thus increasing chances for the onset of diabetes.
本研究旨在确定胰腺组织培养物中念珠菌污染的来源，以及其对胰岛胰岛素分泌活性的影响。胰腺组织取自患有慢性胰腺炎或良性肿瘤的患者的胰腺切除术后。通过手工方法在无菌条件下分离胰岛。采用标准程序进行微生物分析，并在培养的第一天、第三天和第七天测定分泌活性。无菌培养物和感染培养物在培养第一天的胰岛素刺激指数（SI）分别为0.665±0.082和0.982±0.167（以平均值±标准误表示）。在培养第三天，无菌培养物的SI为0.645±0.071，而污染培养物中的这些值更高（1.252±0.413）。在第七天，无菌培养物和感染培养物的SI分别为0.853±0.032和1.239±0.169（P = 0.05）。对培养第一天、第三天和第七天的结果进行分析并比较两组发现，无菌培养物的SI为0.721±0.041，而污染培养物的SI则高出37.68%（SI = 1.157±0.154；P = 0.01）。结果表明，细胞培养污染源于胰腺组织的原发性感染，并且念珠菌可引发此类患者胰岛素分泌水平升高，从而增加糖尿病发病的几率。
