Hansen W A, Christie M R, Kahn R, Norgaard A, Abel I, Petersen A M, Jorgensen D W, Baekkeskov S, Nielsen J H, Lernmark A
Hagedorn Research Laboratory, Niels Steensensvej, Gentofte, Denmark.
Diabetes Res. 1989 Feb;10(2):53-7.
Dithizone, a zinc chelating agent, is known to selectively stain the islets of Langerhans in the pancreas. In the present study, we have used this stain to aid the identification of islets in material obtained by collagenase digestion of human pancreas. Islets were shown to rapidly and reversibly stain red on incubation with dithizone solution. Tissue selected on the basis of dithizone staining was shown to contain insulin-positive cells and to accumulate insulin in the medium during a subsequent period in tissue culture. Experiments with rat islets indicated that the dithizone treatment had no effect on insulin release in tissue culture, on acute responses to stimulatory glucose concentrations or on the insulin content of cells. These results suggest that dithizone staining can assist in the identification of islets from the human pancreas and may prove to be a useful tool in developing techniques for the large scale isolation of functionally intact human islets.
双硫腙是一种锌螯合剂，已知它能选择性地对胰腺中的胰岛进行染色。在本研究中，我们使用这种染色剂来辅助识别通过胶原酶消化人胰腺获得的材料中的胰岛。结果显示，胰岛与双硫腙溶液孵育后会迅速且可逆地染成红色。基于双硫腙染色选择的组织显示含有胰岛素阳性细胞，并且在随后的组织培养期间能在培养基中积累胰岛素。对大鼠胰岛的实验表明，双硫腙处理对组织培养中的胰岛素释放、对刺激葡萄糖浓度的急性反应或细胞的胰岛素含量均无影响。这些结果表明，双硫腙染色可有助于识别来自人类胰腺的胰岛，并且可能被证明是开发大规模分离功能完整的人类胰岛技术的有用工具。
