Key Laboratory of Bio-environmental Engineering, Ministry of Agriculture, China Agricultural University, P.O. Box 67, Beijing 100083, PR China.
Vet J. 2013 Aug;197(2):297-301. doi: 10.1016/j.tvjl.2013.02.007. Epub 2013 Mar 13.
Slightly acidic electrolyzed water (SAEW, pH 5.0-6.5) is a novel disinfectant with environmentally friendly broad spectrum microbial decontamination properties which could have significant utility on farm. Two of the most important pathogenic viruses in pigs are porcine reproductive and respiratory syndrome virus (PRRSV) and pseudorabies virus (PRV). The aim of this study was to evaluate the viricidal effectiveness of SAEW against PRRSV and PRV in vitro under different available chlorine concentrations (ACCs, 30, 50 and 70 mg/L), treatment times (5, 10 and 15 min) and temperatures (4, 20, 40 and 60°C), respectively. SAEW had a strong viricidal activity against both PRRSV and PRV. This activity increased with increasing ACC, treatment time and temperature. PRRSV and PRV titres of 7.0 log(10)TCID(50)/mL and 5.9 log(10)TCID(50)/mL, respectively, were completely inactivated by SAEW at an ACC of ≥ 50 mg/L for 10 min even though SAEW had no negative effect on the host cells. SAEW thus shows promise as a disinfectant for use on pig farms to reduce the spread of both PRRSV and PRV, and to limit the morbidity associated with those viruses.
微酸性电解水（SAEW，pH 值 5.0-6.5）是一种新型消毒剂，具有环保、广谱的微生物消毒性能，在农场中有重要的应用价值。猪的两种最重要的致病性病毒是猪繁殖与呼吸综合征病毒（PRRSV）和猪伪狂犬病病毒（PRV）。本研究旨在评估不同有效氯浓度（30、50 和 70 mg/L）、处理时间（5、10 和 15 min）和温度（4、20、40 和 60°C）下，SAEW 对 PRRSV 和 PRV 的体外杀病毒效果。SAEW 对 PRRSV 和 PRV 均具有很强的杀病毒活性。这种活性随有效氯浓度、处理时间和温度的增加而增加。在有效氯浓度≥50 mg/L、处理 10 min 的条件下，SAEW 可完全灭活 7.0 log(10)TCID(50)/mL 的 PRRSV 和 5.9 log(10)TCID(50)/mL 的 PRV，而对宿主细胞无不良影响。因此，SAEW 有望成为一种消毒剂，用于减少猪场内 PRRSV 和 PRV 的传播，并降低与这些病毒相关的发病率。
