Department of Environmental Science & Technology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
J Biosci Bioeng. 2010 Sep;110(3):308-13. doi: 10.1016/j.jbiosc.2010.03.012. Epub 2010 Apr 18.
In the current study, the effectiveness of slightly acidic electrolyzed water (SAEW) on an in vitro inactivation of Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Salmonella spp. was evaluated and compared with other sanitizers. SAEW (pH 5.6, 23mg/l available chlorine concentration; ACC; and 940mV oxidation reduction potential; ORP) was generated by electrolysis of dilute solution of HCl (2%) in a chamber of a non-membrane electrolytic cell. One milliliter of bacteria suspension (ca. 10-11 log(10)CFU/ml) was mixed with 9ml of SAEW, strong acidic electrolyzed water (StAEW; ca. 50mg/l ACC), sodium hypochlorite solution (NaOCl; ca.120mg/l ACC) and distilled water (DW) as control and treated for 60s. SAEW effectively reduced the population of E. coli, S. aureus and Salmonella spp. by 5.1, 4.8, and 5.2 log(10)CFU/ml. Although, ACC of SAEW was more than 5 times lower than that of NaOCl solution, they showed no significant bactericidal difference (p>0.05). However, the bactericidal effect of StAEW was significantly higher (p<0.05) than SAEW and NaOCl solution in all cases. When tested with each individual test solution, E. coli, S. aureus and Salmonella spp. reductions were not significantly different (p>0.05). These findings indicate that SAEW with low available chlorine concentration can equally inactivate E. coli, S. aureus and Salmonella spp. as NaOCl solution and therefore SAEW shows a high potential of application in agriculture and food industry as an environmentally friendly disinfection agent.
在本研究中，评估并比较了微酸性电解水（SAEW）对大肠杆菌（E. coli）、金黄色葡萄球菌（S. aureus）和沙门氏菌属（Salmonella spp.）的体外灭活效果，与其他消毒剂进行了比较。SAEW（pH 值为 5.6，有效氯浓度为 23mg/L；ACC；氧化还原电位为 940mV；ORP）是通过在非膜电解槽的腔室内电解稀盐酸（2%）溶液产生的。将 1 毫升细菌悬浮液（约 10-11 log(10)CFU/ml）与 9ml 的 SAEW、强酸性电解水（StAEW；约 50mg/L ACC）、次氯酸钠溶液（NaOCl；约 120mg/L ACC）和蒸馏水（DW）混合作为对照，处理 60s。SAEW 可有效减少 E. coli、S. aureus 和 Salmonella spp. 的数量 5.1、4.8 和 5.2 log(10)CFU/ml。虽然 SAEW 的 ACC 比 NaOCl 溶液低 5 倍以上，但它们在杀菌方面没有显著差异（p>0.05）。然而，StAEW 的杀菌效果在所有情况下均明显高于 SAEW 和 NaOCl 溶液（p<0.05）。当用每种单独的测试溶液进行测试时，E. coli、S. aureus 和 Salmonella spp. 的减少没有显著差异（p>0.05）。这些发现表明，低有效氯浓度的 SAEW 可以与 NaOCl 溶液一样有效地灭活 E. coli、S. aureus 和 Salmonella spp.，因此 SAEW 作为一种环保消毒剂在农业和食品工业中有很高的应用潜力。
