Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan, China.
Int J Oral Sci. 2013 Mar;5(1):37-43. doi: 10.1038/ijos.2013.1. Epub 2013 Mar 15.
Regeneration of periodontal tissue is the most promising method for restoring periodontal structures. To find a suitable bioactive three-dimensional scaffold promoting cell proliferation and differentiation is critical in periodontal tissue engineering. The objective of this study was to evaluate the biocompatibility of a novel porcine acellular dermal matrix as periodontal tissue scaffolds both in vitro and in vivo. The scaffolds in this study were purified porcine acellular dermal matrix (PADM) and hydroxyapatite-treated PADM (HA-PADM). The biodegradation patterns of the scaffolds were evaluated in vitro. The biocompatibility of the scaffolds in vivo was assessed by implanting them into the sacrospinal muscle of 20 New Zealand white rabbits. The hPDL cells were cultured with PADM or HA-PADM scaffolds for 3, 7, 14, 21 and 28 days. Cell viability assay, scanning electron microscopy (SEM), hematoxylin and eosin (H&E) staining, immunohistochemistry and confocal microscopy were used to evaluate the biocompatibility of the scaffolds. In vitro, both PADM and HA-PADM scaffolds displayed appropriate biodegradation pattern, and also, demonstrated favorable tissue compatibility without tissue necrosis, fibrosis and other abnormal response. The absorbance readings of the WST-1 assay were increased with the time course, suggesting the cell proliferation in the scaffolds. The hPDL cells attaching, spreading and morphology on the surface of the scaffold were visualized by SEM, H&E staining, immnuohistochemistry and confocal microscopy, demonstrated that hPDL cells were able to grow into the HA-PADM scaffolds and the amount of cells were growing up in the course of time. This study proved that HA-PADM scaffold had good biocompatibility in animals in vivo and appropriate biodegrading characteristics in vitro. The hPDL cells were able to proliferate and migrate into the scaffold. These observations may suggest that HA-PADM scaffold is a potential cell carrier for periodontal tissue regeneration.
牙周组织再生是恢复牙周结构最有前途的方法。在牙周组织工程中，寻找一种合适的生物活性三维支架来促进细胞增殖和分化是至关重要的。本研究旨在评估新型猪去细胞真皮基质作为牙周组织支架的体外和体内生物相容性。本研究中的支架为纯化的猪去细胞真皮基质（PADM）和羟基磷灰石处理的 PADM（HA-PADM）。体外评估了支架的生物降解模式。通过将支架植入 20 只新西兰白兔的骶棘肌中来评估支架的体内生物相容性。将 hPDL 细胞与 PADM 或 HA-PADM 支架共培养 3、7、14、21 和 28 天。采用细胞活力测定、扫描电子显微镜（SEM）、苏木精和伊红（H&E）染色、免疫组织化学和共聚焦显微镜评估支架的生物相容性。体外，PADM 和 HA-PADM 支架均显示出适当的生物降解模式，并且没有组织坏死、纤维化和其他异常反应，表现出良好的组织相容性。随着时间的推移，WST-1 测定的吸光度读数增加，提示支架内细胞增殖。SEM、H&E 染色、免疫组织化学和共聚焦显微镜观察到 hPDL 细胞在支架表面附着、伸展和形态，表明 hPDL 细胞能够生长到 HA-PADM 支架中，并且细胞数量随着时间的推移而增加。本研究证明 HA-PADM 支架在体内具有良好的动物生物相容性和适当的体外生物降解特性。hPDL 细胞能够增殖并迁移到支架中。这些观察结果可能表明 HA-PADM 支架是一种有潜力的牙周组织再生细胞载体。
