Sampsell-Barron Tori
Life Technologies, Madison, WI, USA.
Methods Mol Biol. 2013;1001:81-97. doi: 10.1007/978-1-62703-363-3_8.
Human pluripotent stem cells (hPSCs), including embryonic stem (ES) and induced pluripotent stem (iPS) cells, have been historically cultured in media containing xenogeneic animal components. As hPSC-derived cells and tissues are being developed for human therapies, the application of culture systems to reduce potential immunoreactivity and improve reproducibility becomes increasingly vital. Methods for directly adapting hPSCs to a commercially available culture system free of nonhuman proteins (xeno-free) are described in this chapter.
人类多能干细胞（hPSC），包括胚胎干细胞（ES）和诱导多能干细胞（iPS），一直以来都在含有异种动物成分的培养基中培养。随着源自hPSC的细胞和组织被开发用于人类治疗，应用培养系统以降低潜在免疫反应性并提高可重复性变得愈发重要。本章介绍了将hPSC直接适应不含非人源蛋白的商用培养系统（无血清）的方法。
