Hamill T R, Hamill S G, Busch M P
Irwin Memorial Blood Centers, San Francisco, California.
Transfusion. 1990 May;30(4):302-6. doi: 10.1046/j.1537-2995.1990.30490273435.x.
Experiments were performed to determine the effect of room-temperature (RT) exposure on the growth rates of selected bacteria by deliberate contamination of AS-1 packed red cell (RBC) units contained in PL146 plastic bags. Isolates of Staphylococcus aureus, S. epidermidis, Streptococcus faecalis, Pseudomonas aeruginosa, Citrobacter freundii, Enterobacter agglomerans, and Yersinia enterocolitica were inoculated into 3- to 5-day-old packed RBC units. The units were refrigerated at 4 to 6 degrees C for 14 days. On two occasions during this period, one-half of the units were removed from the refrigerator and held at 25 to 26 degrees C for 2 hours. Timed samples taken during the periods at RT revealed stable or decreasing bacterial counts in the majority of exposed units, and in no instance was a marked increase in bacterial growth observed. Samples taken after subsequent prolonged rerefrigeration showed no significant increase in bacterial counts as compared with those in control units that were refrigerated constantly. These results indicate that RT exposure of contaminated RBC units does not result in accelerated bacterial proliferation over a period of 2 hours and that it may, in fact, have a detrimental effect on bacterial growth.
通过故意污染PL146塑料袋中保存的AS-1浓缩红细胞（RBC）单位，进行实验以确定室温（RT）暴露对选定细菌生长速率的影响。将金黄色葡萄球菌、表皮葡萄球菌、粪肠球菌、铜绿假单胞菌、弗氏柠檬酸杆菌、聚团肠杆菌和小肠结肠炎耶尔森菌的分离株接种到3至5日龄的浓缩RBC单位中。这些单位在4至6摄氏度下冷藏14天。在此期间有两次，将一半的单位从冰箱中取出并在25至26摄氏度下保存2小时。在室温期间定时采集的样本显示，大多数暴露单位中的细菌计数稳定或下降，并且在任何情况下均未观察到细菌生长显著增加。与持续冷藏的对照单位相比，随后长时间重新冷藏后采集的样本中细菌计数没有显著增加。这些结果表明，污染的RBC单位在室温下暴露2小时不会导致细菌加速增殖，实际上可能对细菌生长有不利影响。
