单层离心法优选的猪精子可在体外受精。
Single layer centrifugation-selected boar spermatozoa are capable of fertilization in vitro.
机构信息
Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences, P.O. Box 7054, Uppsala, SE 750 07, Sweden.
出版信息
Acta Vet Scand. 2013 Mar 5;55(1):20. doi: 10.1186/1751-0147-55-20.
BACKGROUND
Good quality spermatozoa are important to achieve fertilization, viable embryos and offspring. Single Layer Centrifugation (SLC) through a colloid (Androcoll-P) selects good quality spermatozoa. However, it has not been established previously whether porcine spermatozoa selected by this method maintain their fertility.
METHODS
The semen was prepared either by SLC or by standard centrifugation (control) and used for in vitro fertilization (IVF) at oocyte:spermatozoa ratios of 1:50; 1:100 and 1:300 (or 4 x 10(3), 8 x 10(3) and 24 x 10(3) spermatozoa/ml) to evaluate their subsequent ability to generate blastocysts. In addition, sperm motility was assessed by computer assisted sperm motility analysis.
RESULTS
Total and progressive motility were significantly higher in sperm samples prepared by SLC compared to uncentrifuged samples. Sperm binding ability, polyspermy, cleavage and blastocyst rates were affected by the oocyte:sperm ratio, but not by sperm treatment.
CONCLUSION
The use of SLC does not adversely affect the in vitro fertilizing and embryo-generating ability of the selected spermatozoa compared to their unselected counterparts, but further modifications in the IVF conditions would be needed to improve the monospermy in IVF systems. Since SLC did not appear to have a negative effect on sperm fertilizing ability, and may in fact select for spermatozoa with a greater potential for fertilization, an in vivo trial to determine the usefulness of this sperm preparation technique prior to artificial insemination is warranted.
背景
优质精子对于实现受精、有活力的胚胎和后代至关重要。单层离心（SLC）通过胶体（Androcoll-P）选择优质精子。然而，之前尚未确定通过这种方法选择的猪精子是否保持其生育能力。
方法
精液通过 SLC 或标准离心（对照）制备，并在卵母细胞：精子比例为 1:50；1:100 和 1:300（或 4 x 10(3), 8 x 10(3) 和 24 x 10(3) 精子/ml）下用于体外受精（IVF），以评估其随后产生囊胚的能力。此外，通过计算机辅助精子运动分析评估精子运动能力。
结果
与未离心的样本相比，通过 SLC 制备的精子样本的总运动和前向运动显著更高。精子结合能力、多精受精、卵裂和囊胚率受卵母细胞：精子比例的影响，但不受精子处理的影响。
结论
与未选择的精子相比，SLC 的使用不会对选择的精子的体外受精和胚胎生成能力产生不利影响，但需要进一步修改 IVF 条件以提高 IVF 系统中的单精受精率。由于 SLC 似乎对精子受精能力没有负面影响，并且实际上可能选择具有更大受精潜力的精子，因此在人工授精前进行体内试验以确定这种精子制备技术的有用性是合理的。
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