Methods for analysis of calcium/calmodulin signaling in cilia and flagella.

The axonemal microtubules of cilia/flagella act as a scaffold for assembly of the protein complexes that ultimately regulate dynein activity to control the size and shape of ciliary bends. Despite our general understanding of the contribution of microtubule sliding to ciliary and flagellar motility, many questions regarding the regulation of dynein remain unanswered. For example, we know that the second messenger calcium plays an important role in modulating dynein activity in response to extracellular cues, but it remains unclear how calcium-binding proteins anchored to the axoneme contribute to this regulation. Recent work has focused on determining the identity and specific functions of these axonemal calcium-binding proteins. Here, we review our current knowledge of calcium-mediated motility and highlight key experiments that have substantially aided our understanding of calcium signaling within the axoneme.