利用SNAP技术分析纤毛膜蛋白动力学
Analysis of ciliary membrane protein dynamics using SNAP technology.
作者信息
Follit John A, Pazour Gregory J
机构信息
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
出版信息
Methods Enzymol. 2013;524:195-204. doi: 10.1016/B978-0-12-397945-2.00011-1.
Abstract
The sensory functions of the primary cilium rely on receptors and other membrane proteins that are specifically sorted to the ciliary compartment, which is a subdomain of the plasma membrane. Defects in this process underlie a large number of human diseases, yet it is poorly understood. Thus, it is of great interest to understand the mechanisms by which the cell sorts and traffics proteins to the ciliary membrane. Here, we provide an overview of our method to study the sorting and trafficking of ciliary membrane proteins using SNAP technology. This technology enables pulse-chase analysis of the movement of proteins through the endomembrane system and onto the cilium.
摘要
初级纤毛的感觉功能依赖于受体和其他膜蛋白，这些蛋白被特异性地分选到纤毛区室，纤毛区室是质膜的一个亚结构域。这一过程中的缺陷是大量人类疾病的基础，但人们对此了解甚少。因此，了解细胞将蛋白质分选并运输到纤毛膜的机制具有重要意义。在这里，我们概述了我们使用SNAP技术研究纤毛膜蛋白分选和运输的方法。该技术能够对蛋白质通过内膜系统并到达纤毛的运动进行脉冲追踪分析。
相似文献
1
Analysis of ciliary membrane protein dynamics using SNAP technology.利用SNAP技术分析纤毛膜蛋白动力学
Methods Enzymol. 2013;524:195-204. doi: 10.1016/B978-0-12-397945-2.00011-1.
2
The cytoplasmic tail of fibrocystin contains a ciliary targeting sequence.纤维囊性蛋白的细胞质尾部含有一个纤毛靶向序列。
J Cell Biol. 2010 Jan 11;188(1):21-8. doi: 10.1083/jcb.200910096. Epub 2010 Jan 4.
3
The Golgin GMAP210/TRIP11 anchors IFT20 to the Golgi complex.高尔基体蛋白GMAP210/TRIP11将IFT20锚定到高尔基体复合体上。
PLoS Genet. 2008 Dec;4(12):e1000315. doi: 10.1371/journal.pgen.1000315. Epub 2008 Dec 26.
4
The intraflagellar transport protein IFT20 is associated with the Golgi complex and is required for cilia assembly.鞭毛内运输蛋白IFT20与高尔基体复合体相关，是纤毛组装所必需的。
Mol Biol Cell. 2006 Sep;17(9):3781-92. doi: 10.1091/mbc.e06-02-0133. Epub 2006 Jun 14.
5
Export of membrane proteins from the Golgi complex to the primary cilium requires the kinesin motor, KIFC1.膜蛋白从高尔基体复合物向初级纤毛的输出需要动力蛋白 KIFC1。
FASEB J. 2018 Feb;32(2):957-968. doi: 10.1096/fj.201700563R. Epub 2018 Jan 4.
6
Arf4 is required for Mammalian development but dispensable for ciliary assembly.Arf4对哺乳动物发育是必需的，但对纤毛组装并非必需。
PLoS Genet. 2014 Feb 20;10(2):e1004170. doi: 10.1371/journal.pgen.1004170. eCollection 2014 Feb.
7
Polycystin-2 takes different routes to the somatic and ciliary plasma membrane.多囊蛋白-2 有不同的途径到达体细胞和纤毛质膜。
J Cell Biol. 2011 Feb 21;192(4):631-45. doi: 10.1083/jcb.201007050. Epub 2011 Feb 14.
8
The Rilp-like proteins Rilpl1 and Rilpl2 regulate ciliary membrane content.Rilp 样蛋白 Rilpl1 和 Rilpl2 调节纤毛膜的内容物。
Mol Biol Cell. 2013 Feb;24(4):453-64. doi: 10.1091/mbc.E12-08-0598. Epub 2012 Dec 21.
9
BLOC-1 is required for selective membrane protein trafficking from endosomes to primary cilia.BLOC-1是内体向初级纤毛进行选择性膜蛋白转运所必需的。
J Cell Biol. 2017 Jul 3;216(7):2131-2150. doi: 10.1083/jcb.201611138. Epub 2017 Jun 2.
10
Membrane proteins follow multiple pathways to the basolateral cell surface in polarized epithelial cells.在极化上皮细胞中，膜蛋白通过多种途径到达基底外侧细胞表面。
J Cell Biol. 2009 Jul 27;186(2):269-82. doi: 10.1083/jcb.200901021. Epub 2009 Jul 20.
引用本文的文献
1
Interactions between TULP3 tubby domain and ARL13B amphipathic helix promote lipidated protein transport to cilia.TULP3 微管域与 ARL13B 两亲性螺旋相互作用促进脂化蛋白向纤毛的转运。
Mol Biol Cell. 2023 Mar 1;34(3):ar18. doi: 10.1091/mbc.E22-10-0473. Epub 2023 Jan 18.
2
Newly synthesized polycystin-1 takes different trafficking pathways to the apical and ciliary membranes.新合成的多囊蛋白-1 采取不同的运输途径到达顶端和纤毛膜。
Traffic. 2018 Dec;19(12):933-945. doi: 10.1111/tra.12612. Epub 2018 Sep 24.
3
Trisomy 21 Represses Cilia Formation and Function.21 三体综合征抑制纤毛的形成和功能。
Dev Cell. 2018 Sep 10;46(5):641-650.e6. doi: 10.1016/j.devcel.2018.07.008. Epub 2018 Aug 9.
4
BLOC-1 is required for selective membrane protein trafficking from endosomes to primary cilia.BLOC-1是内体向初级纤毛进行选择性膜蛋白转运所必需的。
J Cell Biol. 2017 Jul 3;216(7):2131-2150. doi: 10.1083/jcb.201611138. Epub 2017 Jun 2.
本文引用的文献
1
The cytoplasmic tail of fibrocystin contains a ciliary targeting sequence.纤维囊性蛋白的细胞质尾部含有一个纤毛靶向序列。
J Cell Biol. 2010 Jan 11;188(1):21-8. doi: 10.1083/jcb.200910096. Epub 2010 Jan 4.
2
Lateral transport of Smoothened from the plasma membrane to the membrane of the cilium.Smoothened 从质膜侧向转运到纤毛膜。
J Cell Biol. 2009 Nov 2;187(3):365-74. doi: 10.1083/jcb.200907126. Epub 2009 Oct 26.
3
Ciliary biology: understanding the cellular and genetic basis of human ciliopathies.纤毛生物学：了解人类纤毛病的细胞和遗传基础。
Am J Med Genet C Semin Med Genet. 2009 Nov 15;151C(4):263-80. doi: 10.1002/ajmg.c.30227.
4
Targeting proteins to the ciliary membrane.将蛋白质靶向运输至纤毛膜。
Curr Top Dev Biol. 2008;85:115-49. doi: 10.1016/S0070-2153(08)00805-3.
5
Identification of ciliary localization sequences within the third intracellular loop of G protein-coupled receptors.G蛋白偶联受体第三细胞内环内纤毛定位序列的鉴定。
Mol Biol Cell. 2008 Apr;19(4):1540-7. doi: 10.1091/mbc.e07-09-0942. Epub 2008 Feb 6.
6
Human ADPKD primary cyst epithelial cells with a novel, single codon deletion in the PKD1 gene exhibit defective ciliary polycystin localization and loss of flow-induced Ca2+ signaling.在PKD1基因中存在新型单密码子缺失的人类常染色体显性多囊肾病（ADPKD）原发性囊肿上皮细胞表现出纤毛多囊蛋白定位缺陷以及流动诱导的Ca2+信号传导丧失。
Am J Physiol Renal Physiol. 2007 Mar;292(3):F930-45. doi: 10.1152/ajprenal.00285.2006. Epub 2006 Nov 7.
7
Overview of structure and function of mammalian cilia.哺乳动物纤毛的结构与功能概述。
Annu Rev Physiol. 2007;69:377-400. doi: 10.1146/annurev.physiol.69.040705.141236.
8
Polycystin-2 traffics to cilia independently of polycystin-1 by using an N-terminal RVxP motif.多囊蛋白-2通过利用N端RVxP基序独立于多囊蛋白-1转运至纤毛。
J Cell Sci. 2006 Apr 1;119(Pt 7):1383-95. doi: 10.1242/jcs.02818. Epub 2006 Mar 14.
9
Vertebrate Smoothened functions at the primary cilium.脊椎动物的平滑蛋白在初级纤毛处发挥作用。
Nature. 2005 Oct 13;437(7061):1018-21. doi: 10.1038/nature04117. Epub 2005 Aug 31.
10
The vertebrate primary cilium is a sensory organelle.脊椎动物的初级纤毛是一种感觉细胞器。
Curr Opin Cell Biol. 2003 Feb;15(1):105-10. doi: 10.1016/s0955-0674(02)00012-1.
Zotero 插件