Follit John A, Pazour Gregory J
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Methods Enzymol. 2013;524:195-204. doi: 10.1016/B978-0-12-397945-2.00011-1.
The sensory functions of the primary cilium rely on receptors and other membrane proteins that are specifically sorted to the ciliary compartment, which is a subdomain of the plasma membrane. Defects in this process underlie a large number of human diseases, yet it is poorly understood. Thus, it is of great interest to understand the mechanisms by which the cell sorts and traffics proteins to the ciliary membrane. Here, we provide an overview of our method to study the sorting and trafficking of ciliary membrane proteins using SNAP technology. This technology enables pulse-chase analysis of the movement of proteins through the endomembrane system and onto the cilium.
初级纤毛的感觉功能依赖于受体和其他膜蛋白，这些蛋白被特异性地分选到纤毛区室，纤毛区室是质膜的一个亚结构域。这一过程中的缺陷是大量人类疾病的基础，但人们对此了解甚少。因此，了解细胞将蛋白质分选并运输到纤毛膜的机制具有重要意义。在这里，我们概述了我们使用SNAP技术研究纤毛膜蛋白分选和运输的方法。该技术能够对蛋白质通过内膜系统并到达纤毛的运动进行脉冲追踪分析。
