Nassau E, Parsons E R, Johnson G D
J Immunol Methods. 1975 Jan;6(3):261-71. doi: 10.1016/0022-1759(75)90069-1.
The sandwich solid phase radioimmunoassay procedure has been adapted to the serological diagnosis of tuberculosis by the use of disposable plastic haemagglutination trays in which the wells were sensitized with soluble mycobacterial antigen and the subsequent uptake of globulin from test sera detected with radioiodinated antiglobulin. The influence of time and temperature of the incubation stages, pH, washing times, drying and the efficiency of various non-specific blocking agents have been studied. In an initial survey carried out on 319 sera the results obtained showed a good correlation with the presence or absence of established tuberculous infection.
通过使用一次性塑料血凝板，夹心固相放射免疫测定法已被应用于结核病的血清学诊断。在该方法中，血凝板的孔用可溶性分枝杆菌抗原致敏，然后用放射性碘化抗球蛋白检测测试血清中球蛋白的摄取情况。研究了孵育阶段的时间和温度、pH值、洗涤次数、干燥以及各种非特异性封闭剂的效果的影响。在对319份血清进行的初步调查中，所得结果与是否存在已确诊的结核感染有良好的相关性。
