Plaskin D Iu, Ermolin G A, Shipina L K, Vladimirskiĭ M A
Zh Mikrobiol Epidemiol Immunobiol. 1988 Aug(8):79-82.
A solid-phase enzyme immunoassay system for the determination of antibodies to mycobacterial antigens, based on the method of erythrocyte immunoadsorption in microchambers for immunological reactions, has been developed. To detect antibodies specifically bound with the solid-phase antigen, the affinity rosettes of Staphylococcus aureus strain Cowan I, carrying protein A, with erythrocytes conjugated with human gamma globulin have been used. The significant correlation of the titers of 34 sera, determined by means of erythrocyte immunoadsorption, with extinction values obtained in the solid-phase enzyme immunoassay of antibodies to Mycobacterium tuberculosis has been established. The coincidence of the results in 92% of cases has been noted.
基于微室中红细胞免疫吸附用于免疫反应的方法，开发了一种用于测定抗分枝杆菌抗原抗体的固相酶免疫分析系统。为了检测与固相抗原特异性结合的抗体，使用了携带蛋白A的金黄色葡萄球菌考恩I菌株与与人γ球蛋白偶联的红细胞形成的亲和玫瑰花结。已确定通过红细胞免疫吸附测定的34份血清滴度与结核分枝杆菌抗体固相酶免疫分析中获得的吸光度值之间存在显著相关性。已注意到92%的病例结果一致。
