Tufts University School of Medicine, Department of Anatomy & Cellular Biology, Program in Cell, Molecular and Developmental Biology, 150 Harrison Avenue, Boston, MA 02111, USA.
Dev Biol. 2013 Jun 1;378(1):38-50. doi: 10.1016/j.ydbio.2013.02.016. Epub 2013 Mar 7.
The Drosophila wing imaginal disc is subdivided along the proximodistal axis into the distal pouch, the hinge, the surrounding pleura, and the notum. While the genetic pathways that specify the identity of each of these domains have been well studied, the mechanisms that coordinate the relative expansion of these domains are not well understood. Here we investigated the role of the stat92E signal transducer and activator of transcription in wing proximodistal development. We find that stat92E is active ubiquitously in early wing imaginal discs, where it acts to inhibit the induction of ectopic wing fields. Subsequently, stat92E activity is down regulated in the notum and distal pouch. These dynamics coincide with and contribute to the proportional subdivision and expansion of these primordia. As development proceeds, stat92E activity becomes restricted to the hinge, where it promotes normal expansion of the hinge, and restricts expansion of the notum. We also find that stat92E is required autonomously to specify dorsal pleura identity and inhibit notum identity to properly subdivide the body wall. Our data suggest that stat92E activity is regulated along the proximodistal axis to pattern this axis and control the relative expansion of the pouch, hinge, and notum.
果蝇翅膀的胚盘沿着近远轴被细分为远端囊、枢纽、周围胸膜和背板。虽然指定每个这些区域的身份的遗传途径已经得到了很好的研究，但是协调这些区域的相对扩张的机制还没有很好地理解。在这里，我们研究了信号转导和转录激活因子 stat92E 在翅膀近远轴发育中的作用。我们发现，stat92E 在早期翅膀的胚盘中普遍活跃，它抑制了异位翅膀区域的诱导。随后，stat92E 的活性在背板和远端囊中下调。这些动态与这些原基的比例细分和扩张相吻合，并有助于其进行。随着发育的进行，stat92E 的活性被限制在枢纽中，在那里它促进了枢纽的正常扩张，并限制了背板的扩张。我们还发现，stat92E 自主地需要指定背侧胸膜的身份，并抑制背板的身份，以正确地细分体壁。我们的数据表明，stat92E 的活性沿着近远轴被调节，以塑造这个轴，并控制囊、枢纽和背板的相对扩张。
