Department of Anatomy & Cellular Biology, Tufts University School of Medicine, USA.
Mech Dev. 2012 Jul;129(5-8):147-61. doi: 10.1016/j.mod.2012.05.001. Epub 2012 May 14.
The growth and patterning of Drosophila wing and notum primordia depend on their subdivision into progressively smaller domains by secreted signals that emanate from localized sources termed organizers. While the mechanisms that organize the wing primordium have been studied extensively, those that organize the notum are incompletely understood. The genes odd-skipped (odd), drumstick (drm), sob, and bowl comprise the odd-skipped family of C(2)H(2) zinc finger genes, which has been implicated in notum growth and patterning. Here we show that drm, Bowl, and eyegone (eyg), a gene required for notum patterning, accumulate in nested domains in the anterior notum. Ectopic drm organized the nested expression of these anterior notum genes and downregulated the expression of posterior notum genes. The cell-autonomous induction of Bowl and Eyg required bowl, while the non-autonomous effects were independent of bowl. The homeodomain protein Bar is expressed along the anterior border of the notum adjacent to cells expressing the Notch (N) ligand Delta (Dl). bowl was required to promote Bar and repress Dl expression to pattern the anterior notum in a cell-autonomous manner, while lines acted antagonistically to bowl posterior to the Bowl domain. Our data suggest that the odd-skipped genes act at the anterior notum border to organize the notum anterior-posterior (AP) axis using both autonomous and non-autonomous mechanisms.
果蝇翅膀和背板原基的生长和模式形成依赖于其通过从称为组织者的局部来源发出的分泌信号逐渐划分为更小的区域。虽然已经广泛研究了组织翅膀原基的机制，但对于组织背板的机制还不完全了解。odd-skipped（odd）、drumstick（drm）、sob 和 bowl 基因组成了 odd-skipped C(2)H(2)锌指基因家族，该基因家族与背板的生长和模式形成有关。在这里，我们表明，drm、Bowl 和 eyegone（eyg），一个参与背板模式形成的基因，在背板的前区积累在嵌套的域中。异位 drm 组织了这些前背板基因的嵌套表达，并下调了后背板基因的表达。Bowl 和 Eyg 的细胞自主诱导需要 bowl，而非自主效应则独立于 bowl。同源域蛋白 Bar 沿背板的前边界表达，紧邻表达 Notch（N）配体 Delta（Dl）的细胞。bowl 以细胞自主的方式促进 Bar 和抑制 Dl 表达来模式化前背板，而 lines 则在 Bowl 域之后与 bowl 拮抗作用。我们的数据表明，odd-skipped 基因在前背板边界处起作用，使用自主和非自主机制来组织背板的前后（AP）轴。
