眼组织的外显子水平表达谱分析。
Exon-level expression profiling of ocular tissues.
机构信息
Department of Biomedical Engineering, University of Iowa, Iowa City, IA 52242, USA.
出版信息
Exp Eye Res. 2013 Jun;111:105-11. doi: 10.1016/j.exer.2013.03.004. Epub 2013 Mar 14.
Abstract
The normal gene expression profiles of the tissues in the eye are a valuable resource for considering genes likely to be involved with disease processes. We profiled gene expression in ten ocular tissues from human donor eyes using Affymetrix Human Exon 1.0 ST arrays. Ten different tissues were obtained from six different individuals and RNA was pooled. The tissues included: retina, optic nerve head (ONH), optic nerve (ON), ciliary body (CB), trabecular meshwork (TM), sclera, lens, cornea, choroid/retinal pigment epithelium (RPE) and iris. Expression values were compared with publically available Expressed Sequence Tag (EST) and RNA-sequencing resources. Known tissue-specific genes were examined and they demonstrated correspondence of expression with the representative ocular tissues. The estimated gene and exon level abundances are available online at the Ocular Tissue Database.
摘要
眼部组织的正常基因表达谱是研究可能与疾病过程相关基因的宝贵资源。我们使用 Affymetrix Human Exon 1.0 ST 阵列对来自人类供体眼的十种眼部组织进行了基因表达谱分析。从六个不同的个体中获得了十种不同的组织，并对 RNA 进行了混合。这些组织包括：视网膜、视神经头（ONH）、视神经（ON）、睫状体（CB）、小梁网（TM）、巩膜、晶状体、角膜、脉络膜/视网膜色素上皮（RPE）和虹膜。将表达值与公开的表达序列标签（EST）和 RNA 测序资源进行了比较。检查了已知的组织特异性基因，它们的表达与代表性眼部组织相对应。估计的基因和外显子水平丰度可在眼组织数据库在线获得。
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