Ibrahim Ima-Nurisa, Shimizu Kenta, Yoshimatsu Kumiko, Yunianto Andre, Salwati Ervi, Yasuda Shumpei P, Koma Takaaki, Endo Rika, Arikawa Jiro
National Institute of Health Research and Development, Ministry of Health, Jl. Percetakan Negra 29, Jakarta 10560, Indonesia.
J Vet Med Sci. 2013;75(8):1003-8. doi: 10.1292/jvms.12-0442. Epub 2013 Mar 18.
Hemorrhagic fever with renal syndrome (HFRS) is a rodent-borne zoonotic disease caused by hantavirus infection. Many HFRS cases have been reported in East Asia and North Europe, while the situation in Southeast Asia remains unclear. In this study, the prevalence of hantavirus infection in rodents and humans in Thousand Islands regency, which is close to the port of Jakarta, one of the largest historic ports in Indonesia, was investigated. A total of 170 rodents were captured in 2005, and 27 (15.9%) of the rodents were antibody-positive against Hantaan virus antigen in an immunofluorescence assay (IFA) and Western blotting. Despite the high prevalence in rodents, human sera collected from 31 patients with fever of unknown origin and 20 healthy volunteers in the islands in 2009 did not show positive reaction to the antigen in IFA. To identify the virus in rodents genetically, a total of 59 rodents were captured in 2009. Sera from the rodents were screened for antibody by ELISA, and lung tissues were subjected to RT-PCR. 20 (33.9%) of the 59 rodents were antibody-positive, and 3 of those 20 rodents were positive for S and M genome segments of hantaviruses. Genetic analysis showed that the viruses belonged to Seoul virus and formed a cluster with those in Vietnam and Singapore. These results suggest that a unique group of Seoul viruses has spread widely in Southeast Asia.
肾综合征出血热（HFRS）是一种由汉坦病毒感染引起的鼠传人畜共患病。东亚和北欧已报告了许多HFRS病例，而东南亚的情况仍不明朗。在本研究中，对靠近印度尼西亚最大的历史港口之一雅加达港的千岛摄政区啮齿动物和人类中的汉坦病毒感染率进行了调查。2005年共捕获170只啮齿动物，其中27只（15.9%）在免疫荧光试验（IFA）和蛋白质印迹法中对汉滩病毒抗原呈抗体阳性。尽管啮齿动物中的感染率很高，但2009年从该岛屿31名不明原因发热患者和20名健康志愿者采集的人类血清在IFA中对抗原未显示阳性反应。为了从基因上鉴定啮齿动物中的病毒，2009年共捕获59只啮齿动物。通过ELISA对啮齿动物的血清进行抗体筛查，并对肺组织进行逆转录聚合酶链反应（RT-PCR）。59只啮齿动物中有20只（33.9%）抗体呈阳性，其中20只中的3只对汉坦病毒的S和M基因组片段呈阳性。基因分析表明，这些病毒属于汉城病毒，并与越南和新加坡的病毒形成一个聚类。这些结果表明，一组独特的汉城病毒已在东南亚广泛传播。
