Department of Basic Sciences (Microbiology Section), Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan 173230, HP, India.
Arch Microbiol. 2013 May;195(5):357-69. doi: 10.1007/s00203-013-0881-y. Epub 2013 Mar 16.
Two hundred and six phosphate-solubilizing rhizobacteria (PSB) were isolated from rhizosphere soil (RS) and root endosphere (ER) of apple trees from different sites of four locations viz., Chamba, Shimla, Kinnaur and Kullu of Himachal Pradesh, Northern India, and were screened for plant growth promoting traits (PGPTs) by using culture dependent procedures. Indole acetic acid (IAA) production was detected in 50 isolates (24.2 %), siderophore synthesis in 53 isolates (25.7 %), hydrocyanic acid (HCN) in 40 isolates (19.4 %) and percentage growth inhibition against Dematophora necatrix in 61 isolates (29.6 %). Overall, 54.3 % of PSB isolates from RS and 64.4 % from ER showed none of the PGPTs tested. Among the PSB showing PGPTs, 10.6 % had single trait and 30.6 % had multiple traits showing two (10.7 %), three (14.1 %) and four (5.8 %) types of PGPTs. The Shannon-Weaver diversity index (H') revealed that PGPT-possessing PSBs in RS were more abundant than ER. Clustering analysis by principal component analysis showed that ER was most important factor influencing the ecological distribution and physiological characterization of PGPT-possessing PSB. There was a positive correlation (0.94, p < 0.05) between HCN and antifungal activity producers, and IAA and antifungal activity producers (0.99, p < 0.05). Significant positive correlation (0.42, p < 0.05) between HCN producers and altitude was also noted.
从印度北部喜马偕尔邦四个地点（昌巴、希姆拉、金瑙尔和库鲁）不同地点的苹果树根际土壤（RS）和根内区（ER）中分离出 206 株溶磷根际细菌（PSB），并通过依赖培养的程序筛选其具有植物生长促进特性（PGPTs）。在 50 株分离株（24.2%）中检测到吲哚乙酸（IAA）的产生，在 53 株分离株（25.7%）中检测到铁载体合成，在 40 株分离株（19.4%）中检测到氢氰酸（HCN），在 61 株分离株（29.6%）中对Demato phora necatrix的生长抑制率。总体而言，来自 RS 的 PSB 分离株中 54.3%和来自 ER 的 PSB 分离株中 64.4%未显示出任何测试的 PGPTs。在表现出 PGPTs 的 PSB 中，有 10.6%具有单个特性，有 30.6%具有多种特性，表现出两种（10.7%）、三种（14.1%）和四种（5.8%）类型的 PGPTs。Shannon-Weaver 多样性指数（H'）表明，具有 PGPTs 的 RS 中的 PSB 比 ER 更为丰富。通过主成分分析的聚类分析表明，ER 是影响具有 PGPTs 的 PSB 生态分布和生理特征的最重要因素。HCN 和抗真菌活性产生菌之间存在正相关（0.94，p<0.05），IAA 和抗真菌活性产生菌之间也存在正相关（0.99，p<0.05）。还注意到 HCN 产生菌与海拔之间存在显著正相关（0.42，p<0.05）。
