Department of Biotechnology, P.D. Patel Institute of Applied Sciences, Charotar University of Science and Technology, CHARUSAT Campus, Changa 388421, Anand (Gujarat), India.
Microbiol Res. 2014 Jan 20;169(1):66-75. doi: 10.1016/j.micres.2013.07.004. Epub 2013 Jul 26.
Rhizosphere of a halotolerant plant Suaeda fruticosa from saline desert of Little Rann of Kutch, Gujarat (India) was explored for isolation of PGPR form the rare ecological niche having4.33% salinity. Total 85 isolates from the rhizosphere belonging to different species were isolated. Out of 85 isolates, 23 could solubilize phosphate and 11 isolates produced IAA. Seven isolates showed both the traits of phosphate solubilization and IAA production. All isolates which showed either of IAA production or phosphate solubilization or both were further screened for other PGP traits like production of ammonia, siderophore, chitinase, HCN and assessment of their antifungal activity. Out of all the screened isolates, Bacillus licheniformis strain A2 showed most prominent PGP traits in vitro and it was tested in vivo for growth promotion of Groundnut (Arachis hypogaea) under saline soil condition. In presence of soil supplemented with 50 mM NaCl, B. licheniformis treated plants showed increase in fresh biomass, total length and root length by 28%, 24% and 17% and in absence of NaCl it was 43%, 31% and 39% respectively.
从印度古吉拉特邦小拉恩沙漠的耐盐植物滨藜（Suaeda fruticosa）的根际中，探索了从 4.33%盐度的稀有生态位中分离出 PGPR 的方法。从根际中共分离出 85 株属于不同种的细菌。在这 85 株细菌中，有 23 株可以溶解磷酸盐，11 株可以产生 IAA。有 7 株同时表现出溶解磷酸盐和产生 IAA 的特性。所有表现出产生 IAA、溶解磷酸盐或两者兼具的细菌，都进一步筛选了其他 PGP 特性，如氨、铁载体、几丁质酶、HCN 的产生以及对其抗真菌活性的评估。在所筛选的所有菌株中，地衣芽孢杆菌菌株 A2 在体外表现出最显著的 PGP 特性，并在体内对花生（Arachis hypogaea）在盐渍土壤条件下的生长促进作用进行了测试。在添加 50mM NaCl 的土壤中，处理过的地衣芽孢杆菌植物的鲜重生物量、总长度和根长分别增加了 28%、24%和 17%，而在没有 NaCl 的情况下，它们分别增加了 43%、31%和 39%。
