Imperial College London, Silwood Park Campus, Buckhurst Road, Ascot SL5 7PY, UK.
Syst Biol. 2013 Jul;62(4):539-54. doi: 10.1093/sysbio/syt018. Epub 2013 Mar 16.
Guenons (tribe Cercopithecini) are one of the most diverse groups of primates. They occupy all of sub-Saharan Africa and show great variation in ecology, behavior, and morphology. This variation led to the description of over 60 species and subspecies. Here, using next-generation DNA sequencing (NGS) in combination with targeted DNA capture, we sequenced 92 mitochondrial genomes from museum-preserved specimens as old as 117 years. We infer evolutionary relationships and estimate divergence times of almost all guenon taxa based on mitochondrial genome sequences. Using this phylogenetic framework, we infer divergence dates and reconstruct ancestral geographic ranges. We conclude that the extraordinary radiation of guenons has been a complex process driven by, among other factors, localized fluctuations of African forest cover. We find incongruences between phylogenetic trees reconstructed from mitochondrial and nuclear DNA sequences, which can be explained by either incomplete lineage sorting or hybridization. Furthermore, having produced the largest mitochondrial DNA data set from museum specimens, we document how NGS technologies can "unlock" museum collections, thereby helping to unravel the tree-of-life.
长尾猴（Cercopithecini 族）是灵长类动物中最多样化的群体之一。它们分布在整个撒哈拉以南非洲地区，在生态、行为和形态上表现出巨大的变异性。这种变异性导致了超过 60 个种和亚种的描述。在这里，我们使用下一代 DNA 测序（NGS）与靶向 DNA 捕获相结合，对来自博物馆保存的标本进行了 92 个线粒体基因组的测序，这些标本的历史可以追溯到 117 年前。我们根据线粒体基因组序列推断进化关系并估计几乎所有长尾猴类群的分歧时间。利用这个系统发育框架，我们推断分歧日期并重建祖先的地理范围。我们的结论是，长尾猴的非凡辐射是一个复杂的过程，其驱动力包括非洲森林覆盖的局部波动等因素。我们发现从线粒体和核 DNA 序列重建的系统发育树之间存在不一致性，这可以用不完全谱系分选或杂交来解释。此外，通过从博物馆标本中产生最大的线粒体 DNA 数据集，我们记录了 NGS 技术如何“解锁”博物馆藏品，从而有助于揭示生命之树。
