BioElectronics Programme, Institute of Microelectronics, A*STAR (Agency for Science, Technology and Research), 1 Science Park Road, Singapore, 117528, Singapore.
Division of Microelectronics, School of Electrical and Electronics Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore.
Biomed Microdevices. 2013 Aug;15(4):699-709. doi: 10.1007/s10544-013-9757-9.
Our current understanding of clinical significance or the lack thereof of circulating tumor cells (CTCs) is biased by the technology used to isolate these rare cells. Despite the presence of a vast number of academic and commercial technologies, the lack of a standardized and optimized platform has been widely noted. We present a negative enrichment approach, integrating WBC depletion and chemical-free RBC depletion in the same setup without the need for centrifugation, washing or multiple sample handling steps. This approach achieves an average of >90 % recovery of spiked tumor cells and >99 % total WBC depletion in whole blood across multiple cell lines, in a simple and easy-to-use assay. The results presented herein and ongoing improvements aim to fulfill the need for a highly reliable, unbiased, standardized, and optimized CTC isolation platform, using component technologies that are validated for cell isolation.
我们目前对循环肿瘤细胞（CTC）的临床意义的理解，受到用于分离这些稀有细胞的技术的影响。尽管存在大量的学术和商业技术，但缺乏标准化和优化的平台已被广泛注意到。我们提出了一种负向富集方法，在同一个设置中整合白细胞（WBC）去除和无化学试剂的红细胞（RBC）去除，而不需要离心、洗涤或多个样本处理步骤。这种方法在多个细胞系中实现了平均超过 90%的肿瘤细胞回收率和超过 99%的总白细胞（WBC）去除，且操作简单易用。本文提出的结果和正在进行的改进旨在满足对高度可靠、无偏倚、标准化和优化的 CTC 分离平台的需求，使用经过细胞分离验证的组件技术。
