The Second Department of Endocrinology, Henan Provincial People's Hospital, Zhengzhou Henan Province, China.
Cell Biol Int. 2013 May;37(5):430-5. doi: 10.1002/cbin.10046. Epub 2013 Mar 18.
Selective peroxisome proliferator-activated receptor-γ (PPAR-γ) agonist affects the functions of endothelial progenitor cells (EPCs). This study explores the effect of selective PPAR-γ agonist, pioglitazone, on EPC apoptosis. The cells were cultured and identified via the double staining method in a medium containing different concentrations of pioglitazone. EPC apoptosis was detected by flow cytometry. On Day 7, EPCs engulfed DiL-ac-LDL and FITC-UEA-1, and showed yellow fluorescence in a laser-scanning confocal microscope. EPC apoptosis inhibition was maximal at 50 µmol/L. The ability of pioglitazone to prevent EPC apoptosis may be mediated by the PI3K/Akt signal pathway. The use of thiazolidine two ketone (TZD) to reduce EPC apoptosis may have some potential in treating vascular diseases.
选择性过氧化物酶体增殖物激活受体-γ（PPAR-γ）激动剂影响内皮祖细胞（EPCs）的功能。本研究探讨了选择性 PPAR-γ 激动剂吡格列酮对 EPC 细胞凋亡的影响。采用不同浓度吡格列酮的培养基，通过双染色法培养和鉴定细胞。通过流式细胞术检测 EPC 细胞凋亡。在第 7 天，EPCs 吞噬 DiL-ac-LDL 和 FITC-UEA-1，在激光共聚焦显微镜下呈现黄色荧光。50μmol/L 时 EPC 细胞凋亡抑制作用最大。吡格列酮预防 EPC 细胞凋亡的能力可能是通过 PI3K/Akt 信号通路介导的。使用噻唑烷二酮（TZD）减少 EPC 细胞凋亡可能在治疗血管疾病方面具有一定的潜力。
