Department of Environmental Engineering for Symbiosis, Faculty of Engineering, Soka University, 1-236 Tangi-machi, Hachioji, Tokyo 192-8577, Japan.
Int J Syst Evol Microbiol. 2013 Sep;63(Pt 9):3330-3335. doi: 10.1099/ijs.0.048090-0. Epub 2013 Mar 15.
A rod-shaped, endospore-forming, Gram-reaction-positive bacterium, designated strain TC22-2b(T), was isolated from compost in Tochigi, Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belonged to a cluster comprising species of the genus Paenibacillus and was most closely related to the type strain of Paenibacillus elgii (93.4% similarity). The major cellular fatty acids were C(16:0) (25.5%), iso-C(16:0) (23.6%) and anteiso-C(15:0) (21.5%). The predominant menaquinone was MK-7. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The diamino acid found in the cell wall peptidoglycan was meso-diaminopimelic acid, and the DNA G+C content was 59.1 mol%. The results of physiological and biochemical tests enabled the phenotypic differentiation of strain TC22-2b(T) from the most closely related species with validly published names. Phylogenetic and phenotypic evidence reveals that strain TC22-2b(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus thermoaerophilus sp. nov. is proposed. The type strain of the novel species is TC22-2b(T) ( =DSM 26310(T) =JCM 18657(T)).
一株杆状、产芽孢、革兰氏阳性细菌，命名为 TC22-2b(T) 菌株，从日本栃木县的堆肥中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，该菌株属于芽孢杆菌属的一个聚类，与蜡状芽孢杆菌的模式菌株最为密切相关（相似度为 93.4%）。主要细胞脂肪酸为 C(16:0)（25.5%）、iso-C(16:0)（23.6%）和 anteiso-C(15:0)（21.5%）。主要的menaquinone 是 MK-7。主要的极性脂为双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。细胞壁肽聚糖中的二氨基氨基酸是 meso-二氨基庚二酸，DNA G+C 含量为 59.1 mol%。生理生化试验的结果使 TC22-2b(T) 菌株能够从具有有效发表名称的最密切相关的种中进行表型区分。系统发育和表型证据表明，TC22-2b(T) 菌株代表了芽孢杆菌属的一个新种，建议将其命名为嗜热好氧芽孢杆菌（Paenibacillus thermoaerophilus）。新种的模式菌株是 TC22-2b(T)（=DSM 26310(T)=JCM 18657(T)）。
