Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea.
Phytother Res. 2013 Dec;27(12):1874-82. doi: 10.1002/ptr.4943. Epub 2013 Mar 15.
Ultraviolet (UV) irradiation induces photo-damage of the skin, which in turn causes depletion of the dermal extracellular matrix and chronic alterations in skin structure. Skin wrinkle formations are associated with collagen synthesis and matrix metalloproteinase (MMP) expression. The production of type I procollagen is regulated by transforming growth factor-β1 (TGF-β1) expression; the activation of MMP is also correlated with an increase of interleukin-6 (IL-6). Aloe barbadensis M. (Aloe vera) is widely used in cosmetic and pharmaceutical products. In this study, we examined whether baby aloe shoot extract (BAE, immature aloe extract), which is from the one-month-old shoots of Aloe vera, and adult aloe shoot extract (AE), which is from the four-month-old shoots of Aloe vera, have a protective effect on UVB-induced skin photoaging in normal human dermal fibroblasts (NHDFs). The effects of BAE and AE on UVB-induced photoaging were tested by measuring the levels of reactive oxygen species, MMP-1, MMP-3, IL-6, type I procollagen, and TGF-β1 after UVB irradiation. We found that NHDF cells treated with BAE after UVB-irradiation suppressed MMP-1, MMP-3, and IL-6 levels compared to the AE-treated cells. Furthermore, BAE treatment elevated type I procollagen and TGF-β1 levels. Our results suggest that BAE may potentially protect the skin from UVB-induced damage more than AE.
紫外线（UV）辐射会导致皮肤光损伤，进而导致真皮细胞外基质耗竭和皮肤结构的慢性改变。皮肤皱纹的形成与胶原蛋白合成和基质金属蛋白酶（MMP）表达有关。I 型前胶原的产生受转化生长因子-β1（TGF-β1）表达的调节；MMP 的激活也与白细胞介素-6（IL-6）的增加有关。库拉索芦荟 M.（Aloe vera）广泛用于化妆品和制药产品。在这项研究中，我们研究了婴儿库拉索芦荟嫩枝提取物（BAE，未成熟的芦荟提取物）和成年库拉索芦荟嫩枝提取物（AE，四个月大的库拉索芦荟嫩枝提取物）是否对正常人体真皮成纤维细胞（NHDFs）的 UVB 诱导皮肤光老化具有保护作用。通过测量经 UVB 照射后 NHDF 细胞中活性氧、MMP-1、MMP-3、IL-6、I 型前胶原和 TGF-β1 的水平来检测 BAE 和 AE 对 UVB 诱导的光老化的影响。结果发现，与 AE 处理的细胞相比，经 UVB 照射后用 BAE 处理的 NHDF 细胞可抑制 MMP-1、MMP-3 和 IL-6 的水平。此外，BAE 处理可提高 I 型前胶原和 TGF-β1 的水平。我们的结果表明，与 AE 相比，BAE 可能更能保护皮肤免受 UVB 诱导的损伤。
