Misawa Eriko, Tanaka Miyuki, Saito Marie, Nabeshima Kazumi, Yao Ruiqing, Yamauchi Kouji, Abe Fumiaki, Yamamoto Yuki, Furukawa Fukumi
Food Science & Technology Institute, Morinaga Milk Industry Co., Ltd., Kanagawa, Japan.
Department of Dermatology, Wakayama Medical University, Wakayama, Japan.
Photodermatol Photoimmunol Photomed. 2017 Mar;33(2):101-111. doi: 10.1111/phpp.12286. Epub 2017 Jan 24.
Aloe vera is a traditional medical plant whose gel has been widely used in skin care. Previously, we have identified Aloe sterols from Aloe vera as active ingredients. This study investigated the protective effects of Aloe sterols without polysaccharides, against ultraviolet B (UVB)-induced skin photoaging in mice using Aloe vera gel extract (AVGE) obtained by supercritical fluid extraction.
Aloe vera gel extract was supplemented in the diet (12 or 120 ppm), and HR-1 hairless mice were exposed to UVB irradiation for 7 weeks. Skin measurements and histological and analytical studies were performed.
Repeated UVB irradiation induced rough wrinkling of skin with water content reduction and hyperkeratosis. AVGE administration resulted in the significant improvement of UVB-induced skin dryness, epidermal thickness, and wrinkle formation. The AVGE group also suppressed the degenerations of dermal collagen fibers and the appearance of cutaneous apoptosis cells induced by UVB. Furthermore, AVGE administration reduced the excess elevation of pro-inflammatory cytokines (IL-1β and TNF-α) and matrix metalloproteinases (MMP-2, MMP-9, MMP-12, and MMP-13) in UVB-exposed skin.
The dietary ingestion of Aloe sterols protected against chronic UVB damage in mouse skin, and our results suggest that Aloe sterols may prevent skin photoaging through the anti-inflammation and MMP regulation.
芦荟是一种传统药用植物，其凝胶已广泛应用于皮肤护理。此前，我们已从芦荟中鉴定出芦荟甾醇为活性成分。本研究使用超临界流体萃取获得的芦荟凝胶提取物（AVGE），研究了不含多糖的芦荟甾醇对紫外线B（UVB）诱导的小鼠皮肤光老化的保护作用。
在饮食中添加芦荟凝胶提取物（12或120 ppm），对HR-1无毛小鼠进行7周的UVB照射。进行皮肤测量以及组织学和分析研究。
重复UVB照射导致皮肤粗糙起皱，含水量降低和角化过度。给予AVGE可显著改善UVB诱导的皮肤干燥、表皮厚度和皱纹形成。AVGE组还抑制了UVB诱导的真皮胶原纤维变性和皮肤凋亡细胞的出现。此外，给予AVGE可降低UVB照射皮肤中促炎细胞因子（IL-1β和TNF-α）和基质金属蛋白酶（MMP-2、MMP-9、MMP-12和MMP-13）的过度升高。
饮食摄入芦荟甾醇可保护小鼠皮肤免受慢性UVB损伤，我们的结果表明芦荟甾醇可能通过抗炎和调节MMP来预防皮肤光老化。
