Bacteriology, Agroscope-Research Station Changins-Wädenswil ACW, Federal Department of Economic Affairs, Education and Research (EAER), Wädenswil, Switzerland.
Int J Antimicrob Agents. 2013 Jun;41(6):558-63. doi: 10.1016/j.ijantimicag.2013.01.021. Epub 2013 Mar 15.
Antibiotic resistance concerns have been compounded by a report that soil bacteria can catabolise antibiotics, i.e. break down and use them as a sole carbon source. To date this has not been verified or reproduced, therefore in this study soil bacteria were screened to verify and reproduce this hypothesis. Survival in high concentrations of antibiotics was initially observed; however, on further analysis these bacteria either did not degrade the antibiotics or they used an intrinsic resistance mechanism (β-lactamases) to degrade the β-lactams, as demonstrated by high-performance liquid chromatography. These results did not verify or reproduce the hypothesis that bacteria subsist on antibiotics or catabolise antibiotics as previously reported. This study identified that bacteria with a catabolising phenotype did not degrade streptomycin or trimethoprim and therefore could not utilise the antibiotics as a nutrient source. Therefore, we conclude that soil bacteria do not catabolise antibiotics.
有报道称，土壤细菌可以分解抗生素，即分解并将其作为唯一的碳源来使用，这使得人们对抗生素耐药性的担忧更加复杂。迄今为止，这一说法尚未得到证实或重现，因此，在这项研究中，筛选了土壤细菌以验证和重现这一假设。最初观察到这些细菌在高浓度抗生素中存活；然而，进一步的分析表明，这些细菌要么没有降解抗生素，要么它们使用内在的耐药机制（β-内酰胺酶）来降解β-内酰胺类抗生素，这一点通过高效液相色谱得到了证明。这些结果没有证实或重现之前报道的细菌以抗生素为食或分解抗生素的假设。本研究表明，具有分解代谢表型的细菌不能降解链霉素或甲氧嘧啶，因此不能将抗生素作为营养源加以利用。因此，我们得出结论，土壤细菌不会分解抗生素。
