College of Quartermaster Technology, Jilin University, Changchun, China, 130062.
Food Funct. 2013 Apr 30;4(5):763-9. doi: 10.1039/c3fo00013c.
Furan, a widely used industrial compound, has been found in many heat-treated foods, especially baby food. The presence of furan in food raises concerns about public health. In this study, we evaluated the protective effect of salidroside (SR) against furan-induced hepatocyte damage in mice livers. The in vitro antioxidant effects of SR were also evaluated. The results suggested that furan significantly increased hepatocyte damage, as proven by the increased activities of aminotransferase (AST), alanine aminotransferase (ALT) and levels of direct bilirubin (DBIL). Furan also caused oxidative stress, as evidenced by increased reactive oxygen species (ROS) production as well as malondialdehyde (MDA) levels and glutathione S-transferase (GST) activity in mice livers. Pretreatment with SR markedly attenuated the activities of AST, ALT, GST and the levels of DBIL, ROS, and MDA in a dose-dependent manner. The protective effects of SR against furan-induced hepatocyte damage were due to its excellent ability to scavenge free radicals such as 2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radicals, ˙OH, 2,2-di(4-tert-octylphenyl)-1-picrylhydrazyl radicals and ˙O(2)(-). Thus, SR exerted excellent antioxidant effects, and it may be a novel therapeutic and preventive agent for oxidative stress-related diseases.
呋喃是一种广泛使用的工业化合物，存在于许多热处理食品中，特别是婴儿食品中。食品中呋喃的存在引起了公众健康问题的关注。在这项研究中，我们评估了红景天苷（SR）对小鼠肝脏中呋喃诱导的肝细胞损伤的保护作用。还评估了 SR 的体外抗氧化作用。结果表明，呋喃显著增加了肝细胞损伤，这可以通过增加转氨酶（AST）、丙氨酸转氨酶（ALT）和直接胆红素（DBIL）的活性来证明。呋喃还引起了氧化应激，这可以通过增加活性氧（ROS）的产生以及丙二醛（MDA）水平和谷胱甘肽 S-转移酶（GST）的活性来证明。SR 的预处理以剂量依赖性方式显著减弱了 AST、ALT、GST 以及 DBIL、ROS 和 MDA 的水平的活性。SR 对呋喃诱导的肝细胞损伤的保护作用归因于其清除自由基的能力，例如 2'-偶氮-双（3-乙基苯并噻唑啉-6-磺酸）自由基、˙OH、2,2-二（4-叔辛基苯基）-1-苦基肼自由基和˙O（2）（-）。因此，SR 发挥了出色的抗氧化作用，它可能是一种治疗和预防氧化应激相关疾病的新型药物。
