福斯克提取物影响人乳腺癌细胞 MCF-7 的存活和病毒复制。

Forssk. Extract Affects Human Breast Cancer Cell MCF-7 Survival and Viral Replication.

机构信息

Faculty of Medicine and Surgery, "Kore" University of Enna, Contrada Santa Panasia, 94100 Enna, Italy.

Nacture S.r.l., Spin-Off University of Catania, 95123 Catania, Italy.

出版信息

Cells. 2022 May 19;11(10):1696. doi: 10.3390/cells11101696.

DOI:10.3390/cells11101696

PMID:35626733

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9139723/

Abstract

BACKGROUND

Breast cancer (BC) is the leading cause of death worldwide. The severity of BC strictly depends on the molecular subtype. The less aggressive hormone-positive subtype is treated with adjuvant endocrine therapy (AET), which causes both physical and psychological side effects. This condition strongly impacts the adherence and persistence of AET among oncologic patients. Moreover, viral infections also constitute a serious problem for public health. Despite their efficacy, antiviral agents present several therapeutic limits. Accordingly, in the present work, we investigated the antitumor and antiviral activities of Forssk. (), a parasitic plant, endemic to the Mediterranean basin, traditionally known for its beneficial properties for human health.

METHODS

The MTT assay was carried out to evaluate the cytotoxic effect of leaf extract (OCLE) on human breast cancer cells (MCF-7 and MDA-MB-231) and the primary HFF-1 cell line. The lactic dehydrogenase (LDH) assay was performed on MCF-7 cells to analyze necrotic cell death. The antioxidant effect of OCLE was evaluated by intracellular determination of the reactive oxygen species and thiol groups, by DPPH and ABTS assays. The antiviral activity of OCLE was determined against 1, 9, Human respiratory syncytial virus, type 2 and type 5, B1 (CoxB1) and B3 (CoxB3), type 1 (HSV-1) and type 2 (HSV-2), and β- by the plaque reduction assay.

RESULTS

The extract, after 24 h of incubation, did not affect MDA-MB-231 and HFF-1 cell viability. However, at the same time point, it showed a dose-dependent inhibitory effect on MCF-7 cells, with an increase in LDH release. OCLE exhibited free radical scavenging activity and significantly increased non-protein thiol levels in MCF-7 cells. OCLE effectively inhibited HSV-1, HSV-2, CoxB1, and CoxB3 replication.

CONCLUSIONS

The overall results showed an interesting inhibitory effect of OCLE on both MCF-7 cell survival and viral replication.

摘要

背景

乳腺癌（BC）是全球范围内导致死亡的主要原因。BC 的严重程度严格取决于分子亚型。侵袭性较弱的激素阳性亚型采用辅助内分泌治疗（AET）治疗，这会导致身体和心理副作用。这种情况强烈影响肿瘤患者对 AET 的依从性和持久性。此外，病毒感染也是公共卫生的严重问题。尽管抗病毒药物具有疗效，但它们也存在一些治疗限制。因此，在本研究中，我们研究了 Forssk. （）的抗肿瘤和抗病毒活性，这是一种寄生植物，分布在地中海地区，传统上因其对人类健康的有益特性而闻名。

方法

通过 MTT 法评估 Forssk. 叶提取物（OCLE）对人乳腺癌细胞（MCF-7 和 MDA-MB-231）和原代 HFF-1 细胞系的细胞毒性作用。通过 LDH 测定法分析 MCF-7 细胞中的坏死性细胞死亡。通过细胞内测定活性氧和硫醇基团、DPPH 和 ABTS 测定法评估 OCLE 的抗氧化作用。通过噬斑减少测定法确定 OCLE 对 1、9、人呼吸道合胞病毒、2 型和 5 型、B1（CoxB1）和 B3（CoxB3）、1 型（HSV-1）和 2 型（HSV-2）以及 β-的抗病毒活性。

结果

提取物在孵育 24 小时后不影响 MDA-MB-231 和 HFF-1 细胞活力。然而，在同一时间点，它对 MCF-7 细胞表现出剂量依赖性的抑制作用，同时导致 LDH 释放增加。OCLE 具有清除自由基的活性，并显著增加 MCF-7 细胞中非蛋白硫醇水平。OCLE 有效抑制 HSV-1、HSV-2、CoxB1 和 CoxB3 的复制。