Ankalife IVF and Women Health Centre, Ankara, Turkey.
Biomed Res Int. 2013;2013:529589. doi: 10.1155/2013/529589. Epub 2013 Feb 18.
The recent reports on the treatment of azoospermia patients, in which spermatozoa could not be traced in their testes, are focused more on the potential use of adult stem cells, like mesenchymal stem cells (MSCs). The aim of this study was to demonstrate the potential use of MSCs derived from adipose tissue in the treatment of azoospermia using rat disease models. After busulfan application, the rats (n = 20) were injected with the GFP(+) MSCs into left rete testes. After 12 weeks, the testes with cell injection (right testes) were compared to control (left testes) after dimensional and immunohistochemical analyses. Testes treated with MSCs appeared morphologically normal, but they were atrophic in rats without stem cell treatment, in which the seminiferous tubules were empty. Spermatogenesis was detected, not in every but in some tubules of cell-treated testes. GFP(+)/VASA(+) and GFP(+)/SCP1(+) cells in testes indicated the transdifferentiation of MSCs into spermatogenetic cells in the appropriate microenvironment. Rats with cell treatment were mated to show the full recovery of spermatogenesis, and continuous generations were obtained. The expression of GFP was detected in the mesenchymal stem cells derived from adipose tissue and bone marrow and also in the sperms of offspring. In conclusion, MSCs might be studied for the same purpose in humans in future.
最近关于治疗无精子症患者的报告，这些患者的睾丸中无法追踪到精子，更多地关注了成体干细胞（如间充质干细胞（MSCs））的潜在用途。本研究旨在使用大鼠疾病模型证明来源于脂肪组织的 MSCs 在治疗无精子症中的潜在用途。在应用白消安后，将 GFP(+)MSCs 注射到大鼠左侧网织睾丸中。12 周后，通过尺寸和免疫组织化学分析比较细胞注射的睾丸（右侧睾丸）与对照（左侧睾丸）。接受 MSC 治疗的睾丸形态正常，但在未接受干细胞治疗的大鼠中出现萎缩，其中精小管为空。在细胞处理的睾丸中，并非每个小管都检测到精子发生，但有一些小管中检测到了精子发生。睾丸中 GFP(+)/VASA(+)和 GFP(+)/SCP1(+)细胞表明 MSC 在适当的微环境中向生殖细胞的转分化。接受细胞治疗的大鼠进行交配以显示精子发生的完全恢复，并获得了连续几代。在脂肪组织和骨髓来源的间充质干细胞以及后代的精子中都检测到 GFP 的表达。总之，未来可能会在人类中对 MSCs 进行相同的研究。
