Department of Proteomics and Systems Medicine, BHF Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
Electrophoresis. 2013 Jun;34(11):1452-64. doi: 10.1002/elps.201200708. Epub 2013 May 8.
Proteome analysis using capillary electrophoresis coupled to mass spectrometry (CE-MS) has been used in a number of clinical applications in the past years. The main focus of CE-MS-based studies has been on the investigation of urine, due to the stability of the urinary proteome, ease of collection, and also the low molecular weight range of the urinary proteome, mostly peptides below 30 kDa. The reproducibility of this approach has enabled analysis of over 20 000 samples in a comparable way, giving enormous statistical power to any additional study involving this methodological setup. In this article, we review the major technological issues associated with the application of CE-MS in the routine investigation of the urinary proteome for clinical applications. We pinpoint recent developments that may have a chance to improve on the currently used approach, and highlight obstacles that need to be solved. In the second part of the article, we review the recent clinical applications, aiming to highlight relevant issues, and possible future routine applications in clinical diagnosis. In the end, we provide a short outlook, and indicate future developments to be expected, as well as problems that need to be solved to enable routine application of CE-MS in a clinical setting.
过去几年中，毛细管电泳与质谱联用（CE-MS）的蛋白质组分析已在许多临床应用中得到应用。基于 CE-MS 的研究主要集中在尿液方面，这是由于尿液蛋白质组的稳定性、收集的便利性以及尿液蛋白质组的低分子量范围（主要是低于 30 kDa 的肽）。这种方法的可重复性使我们能够以类似的方式分析超过 20000 个样本，为任何涉及这种方法设置的额外研究提供了巨大的统计能力。在本文中，我们回顾了与 CE-MS 在临床应用中常规研究尿液蛋白质组相关的主要技术问题。我们指出了最近可能改进当前方法的发展，并强调了需要解决的障碍。在文章的第二部分，我们回顾了最近的临床应用，旨在突出相关问题，并可能在未来的临床诊断中常规应用。最后，我们提供了一个简短的展望，并指出了未来的发展方向以及为了使 CE-MS 能够在临床环境中常规应用而需要解决的问题。
