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通过抑制过氧化氢酶打破种子休眠

Breaking of seed dormancy by catalase inhibition.

作者信息

Hendricks S B, Taylorson R B

出版信息

Proc Natl Acad Sci U S A. 1975 Jan;72(1):306-9. doi: 10.1073/pnas.72.1.306.

Abstract

Germination of some dormant seeds is promoted by solutions of thiourea, sodium nitrite, and hydroxylamine salts. The promotions are accompanied by irreversible inhibition of catalase (EC 1.11.1.6) in extracts from the seeds. The seeds are also promoted in germination by catechol and pyrogallol solutions. These effects are recorded for lettuce (Lactuca sativa L. cv. Grand Rapids) and pigweed (Amaranthus albus L.) seeds. The results indicae that metabolically derived hydrogen peroxide, spared from decomposition by catalase inhibition, oxidizes reduced NADPH required as the oxidant in the pentose pathway of glucose use. The metabolic system for such use of H2O2 involves the enzymes, peroxidase (EC 1.11.1.7) and pyridine nucleotide quinone oxidoreductase (EC 1.6.99.2), which are present in the dormant seed prior to imbibition of water.

摘要

硫脲、亚硝酸钠和羟胺盐溶液可促进一些休眠种子的萌发。这种促进作用伴随着种子提取物中过氧化氢酶(EC 1.11.1.6)的不可逆抑制。儿茶酚和连苯三酚溶液也可促进种子萌发。这些效应在生菜(Lactuca sativa L. cv. Grand Rapids)和藜(Amaranthus albus L.)种子中得到记录。结果表明,通过过氧化氢酶抑制作用而免于分解的代谢产生的过氧化氢,氧化了葡萄糖戊糖途径中作为氧化剂所需的还原型NADPH。这种利用过氧化氢的代谢系统涉及过氧化物酶(EC 1.11.1.7)和吡啶核苷酸醌氧化还原酶(EC 1.6.99.2),它们在种子吸水前就存在于休眠种子中。

相似文献

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Breaking of seed dormancy by catalase inhibition.通过抑制过氧化氢酶打破种子休眠
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