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过氧化氢酶(EC 1.11.1.6)活性变化与葡萄(Vitis vinifera L.)芽休眠的关系

Changes in the Activity of Catalase (EC 1.11.1.6) in Relation to the Dormancy of Grapevine (Vitis vinifera L.) Buds.

作者信息

Nir G, Shulman Y, Fanberstein L, Lavee S

机构信息

Department of Olei and Viticulture, Agricultural Research Organization, Volcani Center, Bet Dagan 50-250, Israel.

出版信息

Plant Physiol. 1986 Aug;81(4):1140-2. doi: 10.1104/pp.81.4.1140.

Abstract

Catalase activity in grapevine (Vitis vinifera L.) buds cv. ;Perlette.' increased to a maximum in October and thereafter decreased within 3 months to less than half its maximal rate. The decrease in catalase activity coincided with the decline in temperature during winter. The rate of sprouting of buds forced at 23 degrees C was negatively related to the activity of catalase. Artificial chilling of grapevine canes at 5 degrees C resulted in a 25% decrease of catalase activity in the buds after 3 days and 31% after 17 days. The activity of catalase increased to the control level only 96 hours after removing canes from 5 degrees C to room temperature. Efficient buddormancy breaking agents, such as thiourea and cyanamide decreased catalase activity to 64 and 50% of the controls respectively, while the activity of peroxidase remained the same under those conditions. A less efficient dormancy breaking agent dinitro-ortho-cresol, did not decrease catalase activity.

摘要

葡萄(欧亚种葡萄)品种“佩莱特”芽中的过氧化氢酶活性在10月升至最高,此后在3个月内降至最高速率的一半以下。过氧化氢酶活性的下降与冬季温度的降低同时发生。在23摄氏度催芽的芽的萌发速率与过氧化氢酶活性呈负相关。将葡萄茎在5摄氏度下人工冷藏,3天后芽中的过氧化氢酶活性降低25%,17天后降低31%。从5摄氏度移至室温后,仅96小时过氧化氢酶活性就恢复到对照水平。高效的破眠剂,如硫脲和氰胺,分别将过氧化氢酶活性降低至对照的64%和50%,而在这些条件下过氧化物酶活性保持不变。效率较低的破眠剂二硝基邻甲酚则不会降低过氧化氢酶活性。

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