Schumacher Loni L, Love Brenda C, Ferrell Mark, DeSilva Udaya, Fernando Ruchika, Ritchey Jerry W
Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK 74078, USA.
J Vet Diagn Invest. 2013 Mar;25(2):304-7. doi: 10.1177/1040638713479604.
A 12-year-old intact male Miniature Schnauzer dog with chronic diarrhea that was unresponsive to empirical treatment was presented to a referring veterinarian. A laparotomy was performed, and formalin-fixed biopsies of duodenum, jejunum, and colon were sent to Oklahoma Animal Disease Diagnostic Laboratory for evaluation. Histologic examination revealed a severe, diffuse, granulomatous enteritis and colitis with intralesional yeast and hyphal forms. Grocott methenamine silver stains revealed short, aseptate hyphae co-mingled with 2-8 µm, oval to round yeast organisms consistent with Histoplasma capsulatum. The atypical presentation of both yeast and hyphal forms prompted identification of the organism. Direct sequencing of a polymerase chain reaction product from paraffin-embedded intestinal samples confirmed the presence of Ajellomyces capsulatus with a homology over 99% to several sequences in GenBank. Ajellomyces capsulatus is the holomorphic name for H. capsulatum. Therefore, the mycelial form of a dimorphic fungus such as H. capsulatum can coexist with yeast cells within lesions of histoplasmosis. Following diagnosis, the dog was treated with itraconazole for 6 months and has improved.
一只12岁未绝育的雄性迷你雪纳瑞犬，患有慢性腹泻，经验性治疗无效，被转诊至一位兽医处。进行了剖腹手术，并将十二指肠、空肠和结肠的福尔马林固定活检组织送至俄克拉荷马动物疾病诊断实验室进行评估。组织学检查显示严重的弥漫性肉芽肿性小肠炎和结肠炎，病变内有酵母和菌丝形态。格罗科特六胺银染色显示短的无隔菌丝与2-8微米的椭圆形至圆形酵母样生物体混合，符合荚膜组织胞浆菌。酵母和菌丝形态的非典型表现促使对该生物体进行鉴定。对石蜡包埋的肠道样本进行聚合酶链反应产物的直接测序，证实存在荚膜阿耶洛霉，与GenBank中的几个序列同源性超过99%。荚膜阿耶洛霉是荚膜组织胞浆菌的全型名称。因此，像荚膜组织胞浆菌这样的双相真菌的菌丝形态可以与组织胞浆菌病病变内的酵母细胞共存。诊断后，该犬接受了6个月的伊曲康唑治疗，病情有所改善。
