Pierelli L, Menichella G, Paoloni A, Teofili L, Sica S, Foddai M L, Rossi P L, Leone G, Mango G, Bizzi B
Servizio di Ematologia ed Emotrasfusione, Università Cattolica del Sacro Cuore, Roma, Italy.
Haematologica. 1990 Jan-Feb;75 Suppl 1:29-32.
Twenty collections of peripheral blood stem cells were performed in 3 patients (2 NHL, 1 AML) using the Haemonetics V50S discontinuous flow blood cell separator. A modified lymphocyte collection protocol (Nebraska Surge) was used in all instances. Leukapheresis were performed after 1 or 2 courses of chemotherapy and started when peripheral blood leukocytes count reached 1 x 10e9/l and platelets count 80 x 10e9/l. A mean blood volume of 5.9 +/- 0.6 litres was processed per procedure and the mean yields for mononuclear cells, nucleated cells and CFU-GM were respectively 5.4 +/- 1.4 x 10e9, 4.9 +/- 1.6 x 10e9 and 128.5 +/- 182.3 x 10e4 per procedure. Haemonetics V50S had showed a mean collection efficiency for mononuclear cells of 67.5 +/- 5.0% per procedure. Results obtained are not significantly different from the ones obtained with an automated continuous flow separator even if extracorporeal circulation is consistently high in patients with a low hematocrit when the 250 ml Latham Bowl is used.
使用Haemonetics V50S间断流血细胞分离机对3例患者（2例非霍奇金淋巴瘤、1例急性髓细胞白血病）进行了20次外周血干细胞采集。所有情况下均采用改良的淋巴细胞采集方案（内布拉斯加激增方案）。在1或2个疗程化疗后进行白细胞单采术，当外周血白细胞计数达到1×10⁹/L且血小板计数达到80×10⁹/L时开始。每次操作处理的平均血量为5.9±0.6升，每次操作单核细胞、有核细胞和集落形成单位-粒细胞巨噬细胞的平均产量分别为5.4±1.4×10⁹、4.9±1.6×10⁹和128.5±182.3×10⁴。Haemonetics V50S每次操作单核细胞的平均采集效率为67.5±5.0%。即使在使用250 ml莱瑟姆杯时血细胞比容低的患者体外循环持续较高的情况下，所获得的结果与使用自动连续流分离器获得的结果相比无显著差异。
