用于从血液中采集祖细胞和干细胞的间歇性和连续性流动细胞分离器的患者体内比较。

Intrapatient comparison of an intermittent and a continuous flow cell separator for the collection of progenitor and stem cells from the blood.

作者信息

Beretta F, van den Bosch S, Castelli D, Cavalli F, Ghielmini M

机构信息

Servizio Oncologico Cantonale, Bellinzona, Svizzera.

出版信息

Vox Sang. 1998;75(2):149-53.

PMID:9784670

Abstract

BACKGROUND AND OBJECTIVES

Continuous-flow and intermittent-flow blood cell separators (CFCS and IFCS) are both used to collect stem cells from the blood to rescue patients undergoing myeloablative treatment for cancer.

MATERIALS AND METHODS

We designed a study to compare the collection efficiency of the two systems. The continuous-flow Cobe Spectra and the intermittent-flow Haemonetics MCS-3P were used to collect cells on consecutive days from 9 patients mobilised with G-CSF with or without chemotherapy. Blood obtained before leukapheresis and the leukapheresis product were analysed for their content of red and white cells, platelets, CD34-positive cells, GM-CFC, CFC-E, and BFU-E. An extraction ratio was calculated.

RESULTS

We found that the CFCS extracted about 4 times more mononuclear cells per unit time, 3 times more CD34-positive, and 4 times more clonogenic cells than the IFCS. The subject acceptability of the two systems was similar.

CONCLUSION

The CFCS is a more efficient system for stem cell collection. IFCS requires a longer harvesting time for the same result.

摘要

背景与目的

连续流式和间歇流式血细胞分离机（CFCS和IFCS）均用于从血液中采集干细胞，以挽救接受癌症清髓治疗的患者。

材料与方法

我们设计了一项研究来比较这两种系统的采集效率。连续流式的Cobe Spectra和间歇流式的Haemonetics MCS - 3P被用于连续数天从9名接受G - CSF动员且有或无化疗的患者中采集细胞。对白细胞分离术前采集的血液以及白细胞分离术产品进行红细胞、白细胞、血小板、CD34阳性细胞、粒 - 巨噬细胞集落形成细胞（GM - CFC）、红细胞集落形成细胞（CFC - E）和爆式红系集落形成单位（BFU - E）含量分析，并计算提取率。