Andreu-Ballester Juan C, Tormo-Calandín Constantino, Garcia-Ballesteros Carlos, Pérez-Griera J, Amigó Victoria, Almela-Quilis Amadeo, Ruiz del Castillo Juan, Peñarroja-Otero Carlos, Ballester Ferran
Research Department and Emergency Department, Arnau de Vilanova Hospital, Valencia, Spain.
Clin Vaccine Immunol. 2013 May;20(5):738-46. doi: 10.1128/CVI.00752-12. Epub 2013 Mar 20.
Gamma-delta T cells are the most abundant of all epithelial-resident lymphocytes and are considered a first line of defense against pathogens in the mucosa. Our objective was to confirm the reduction in γδ T cell subsets and its relationship with mortality in patients with sepsis. We studied 135 patients with sepsis attended in the emergency department and intensive care unit of two hospitals and compared them with a similar control group of healthy subjects. The αβ and γδ T cell subsets were determined via flow cytometry according to the stage of the sepsis and its relationship with mortality. All the lymphocyte subsets were reduced with respect to the corresponding subsets in the control group. All the γδ T cell populations decreased significantly as the septic picture worsened. Furthermore, γδ T cells showed decreases at days 2, 3, and 4 from the start of sepsis. Twenty-six patients with sepsis died (19.3%). The γδ T cells, specifically, the CD3(+) CD56(+) γδ T cells, were significantly reduced in those septic patients who died. Our results indicate that, during sepsis, γδ T cells show the largest decrease and this reduction becomes more intense when the septic process becomes more severe. Mortality was associated with a significant decrease in γδ T cells.
γδ T细胞是所有上皮驻留淋巴细胞中数量最多的，被认为是黏膜抵御病原体的第一道防线。我们的目的是证实脓毒症患者γδ T细胞亚群的减少及其与死亡率的关系。我们研究了两家医院急诊科和重症监护病房收治的135例脓毒症患者，并将他们与一组类似的健康对照者进行比较。根据脓毒症的阶段及其与死亡率的关系，通过流式细胞术测定αβ和γδ T细胞亚群。与对照组相应亚群相比，所有淋巴细胞亚群均减少。随着脓毒症病情加重，所有γδ T细胞群体均显著减少。此外，从脓毒症开始第2、3和4天，γδ T细胞数量减少。26例脓毒症患者死亡（19.3%）。具体而言，死亡的脓毒症患者中γδ T细胞，尤其是CD3(+) CD56(+) γδ T细胞显著减少。我们的结果表明，在脓毒症期间，γδ T细胞减少最为明显，且当脓毒症进程加重时，这种减少更为显著。死亡率与γδ T细胞显著减少有关。
