Joh Eun-Ha, Jeong Jin-Ju, Kim Dong-Hyun
Radioisotope Research Division, Department of Research Reactor Utilization, Korea Atomic Energy Research Institute (KAERI), Daejeon, 305-353, South Korea.
Arch Pharm Res. 2014 Feb;37(2):225-31. doi: 10.1007/s12272-013-0092-8. Epub 2013 Mar 21.
The rhizome of Codonopsis lanceolata (family Campanulaceae), which contains lancemaside A as a main constituent, is frequently used in the traditional Chinese medicine for the treatment of inflammatory diseases. Lancemaside A exhibits anti-inflammatory effect in vitro and in vivo. However, orally administered lancemaside A is metabolized to echinocystic acid by the intestinal microflora and the metabolite is absorbed into the blood. Therefore, to understand whether echinocystic acid is effective against skin inflammatory diseases, we assessed its inhibitory effect against 12-O-tetra decanoylphorbol-13-acetate (TPA)-induced ear inflammation in mice. Topically administered echinocystic acid potently suppressed TPA-induced ear swelling. The suppression rates at 0.05 and 0.10 % concentrations were 65 and 73 %, respectively. Echinocystic acid also inhibited TPA-induced myeloperoxidase activity, as well as COX-2, iNOS, TNF-α and IL-1β expressions. Echinocystic acid inhibited NF-κB in TPA-treated mouse ears, as well as in lipopolysaccharide-stimulated peritoneal macrophages. Its potency is comparable with that of dexamethasone. These findings indicate that echinocystic acid may ameliorate inflammatory diseases, such as dermatitis.
轮叶党参（桔梗科）的根茎，其主要成分为轮叶党参苷A，常用于传统中药治疗炎症性疾病。轮叶党参苷A在体外和体内均表现出抗炎作用。然而，口服的轮叶党参苷A会被肠道微生物群代谢为紫锥菊酸，且该代谢产物会被吸收进入血液。因此，为了解紫锥菊酸是否对皮肤炎症性疾病有效，我们评估了其对12-O-十四烷酰佛波醇-13-乙酸酯（TPA）诱导的小鼠耳部炎症的抑制作用。局部应用紫锥菊酸可有效抑制TPA诱导的耳部肿胀。0.05%和0.10%浓度下的抑制率分别为65%和73%。紫锥菊酸还可抑制TPA诱导的髓过氧化物酶活性以及COX-2、iNOS、TNF-α和IL-1β的表达。紫锥菊酸可抑制TPA处理的小鼠耳部以及脂多糖刺激的腹腔巨噬细胞中的NF-κB。其效力与地塞米松相当。这些发现表明紫锥菊酸可能改善炎症性疾病，如皮炎。
