Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science & Technology (KNUST), Kumasi, Ghana.
Department of Pathology, Komfo Anokye Teaching Hospital (KATH), Kumasi, Ghana.
Biomed Res Int. 2018 Jul 24;2018:3984068. doi: 10.1155/2018/3984068. eCollection 2018.
The therapeutic potential of stigmasterol, a natural steroid alcohol with established immune-modulatory properties, was assessed on allergic cutaneous responses. We examined its suppressive effect on immunoglobulin E (IgE)-mediated active cutaneous anaphylaxis (ACA), compound 48/80 (C48/80)-induced pruritus, and irritant dermatitis induced by 12-O-tetradecanoylphorbol-13-acetate (TPA). Stigmasterol at 10-100 mg/kg significantly inhibited ACA with reduction in reaction area and concentration of the extravasated Evans blue dye. Given at 50 and 100 mg/kg, stigmasterol significantly inhibited C48/80-induced scratching behaviour when compared to saline-treated C48/80-injected control. Skin histopathology of injected sites confirmed that stigmasterol reduced mast cell trafficking and degranulation associated with C48/80-induced pruritus. Stigmasterol controlled inflammatory features such as ear skin oedema and neutrophilia and also reduced serum levels of TNF induced by topical application of TPA. Epidermal layer thickening and inflammatory cell infiltration of ear skin tissue were significantly reduced by stigmasterol. Taken together, stigmasterol demonstrates significant potential as a molecule of interest in allergic skin disease therapy.
我们评估了具有既定免疫调节特性的天然甾体醇——豆甾醇在过敏性皮肤反应中的治疗潜力。我们研究了它对免疫球蛋白 E（IgE）介导的主动皮肤过敏反应（ACA）、化合物 48/80（C48/80）诱导的瘙痒以及 12-O-十四烷酰佛波醇-13-乙酸酯（TPA）诱导的刺激性皮炎的抑制作用。豆甾醇在 10-100mg/kg 时显著抑制 ACA，减少了渗出的 Evans 蓝染料的反应面积和浓度。与盐水处理的 C48/80 注射对照相比，当给予 50 和 100mg/kg 时，豆甾醇显著抑制了 C48/80 诱导的搔抓行为。注射部位的皮肤组织病理学证实，豆甾醇减少了肥大细胞的迁移和 C48/80 诱导的瘙痒相关的脱颗粒。豆甾醇控制了炎症特征，如耳部皮肤水肿和嗜中性粒细胞增多，并降低了 TPA 局部应用诱导的 TNF 的血清水平。豆甾醇显著减少了耳部皮肤组织的表皮层增厚和炎症细胞浸润。综上所述，豆甾醇作为一种治疗过敏性皮肤病的候选分子具有显著的潜力。
