特克汀 2 和 CatSper 2 在正常精子和少弱精子症男性中的水平及其与活力、受精率、胚胎质量和妊娠率的关系。
Levels of Tektin 2 and CatSper 2 in normozoospermic and oligoasthenozoospermic men and its association with motility, fertilization rate, embryo quality and pregnancy rate.
机构信息
Jaslok Hospital, 15, Dr G Deshmukh Marg, Mumbai - 26, India.
出版信息
J Assist Reprod Genet. 2013 Apr;30(4):513-23. doi: 10.1007/s10815-013-9972-6. Epub 2013 Mar 22.
PURPOSE
To compare the expression profiles of Tektin 2 and CatSper 2 motility proteins in the spermatozoa of normozoospermic and oligoasthenozoospermic men and determine its correlation with sperm motility, fertilization rate, embryo quality and pregnancy rate.
METHODS
Tektin 2 and CatSper 2 protein expression was studied using Western Blotting and immunofluorescence. Tektin 2 and CatSper 2 protein levels were quantified by ELISA.
RESULTS
Oligoasthenozoospermic men were found to have lower fertilization rates, poor embryo quality and lower pregnancy rates as compared to normozoospermic men. The levels of Tektin 2 and CatSper 2 are significantly lower in spermatozoa of oligoasthenozoospermic men as compared to normozoospermic controls; the levels were also lower in immotile fraction as compared to motile fraction of spermatozoa obtained from normozoospermic individuals. The levels of Tektin 2 and CatSper 2 were higher in individuals demonstrating sperm motility >60 % as compared to sperm motility <30 %. Tektin 2 but not CatSper 2 levels were positively associated with fertilization rate, embryo quality and pregnancy rate.
CONCLUSION
Levels of Tektin 2 and CatSper 2 proteins are positively associated with sperm motility parameters. Measurements of Tektin 2 levels can be correlated with the clinical outcome of ICSI.
目的
比较正常精子和少弱精子症患者精子中 Tektin 2 和 CatSper 2 运动蛋白的表达谱，并确定其与精子运动能力、受精率、胚胎质量和妊娠率的相关性。
方法
使用 Western Blotting 和免疫荧光法研究 Tektin 2 和 CatSper 2 蛋白的表达。使用 ELISA 定量 Tektin 2 和 CatSper 2 蛋白水平。
结果
与正常精子症患者相比，少弱精子症患者的受精率较低，胚胎质量较差，妊娠率较低。与正常精子对照组相比，少弱精子症患者精子中的 Tektin 2 和 CatSper 2 水平明显较低；与正常个体精子的运动部分相比，不动部分的水平也较低。与精子运动率<30%的个体相比，精子运动率>60%的个体的 Tektin 2 和 CatSper 2 水平更高。Tektin 2 水平与受精率、胚胎质量和妊娠率呈正相关，但 CatSper 2 水平则无此相关性。
结论
Tektin 2 和 CatSper 2 蛋白水平与精子运动参数呈正相关。Tektin 2 水平的测量可以与 ICSI 的临床结果相关联。
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