Martínez-Heredia Juan, de Mateo Sara, Vidal-Taboada José M, Ballescà José Luis, Oliva Rafael
Human Genetics Research Group, Genetics Unit, Faculty of Medicine, University of Barcelona, Casanova 143, 08036 Barcelona, Spain.
Hum Reprod. 2008 Apr;23(4):783-91. doi: 10.1093/humrep/den024. Epub 2008 Feb 15.
Asthenozoospermia is one of the most common findings present in infertile males, but its aetiology remains unknown in most cases. Present proteomic tools now offer the opportunity to identify proteins which are differentially expressed in asthenozoospermic semen samples and potentially involved in infertility.
We compared the expression of 101 sperm protein spots in 20 asthenozoospermic samples to that of 10 semen donor controls using two-dimensional proteomic analysis.
Seventeen protein spots have been identified at different amounts in the asthenozoospermic samples compared with controls. These are cytoskeletal actin-B, annexin-A5, cytochrome C oxidase-6B, histone H2A, prolactin-inducible protein and precursor, calcium binding protein-S100A9 (2 spots), clusterin precursor, dihydrolipoamide dehydrogenase precursor, fumarate hydratase precursor, heat shock protein-HSPA2, inositol-1 monophosphatase, 3-mercapto-pyruvate sulfurtransferase/dienoyl-CoA isomerase precursor, proteasome subunit-PSMB3, semenogelin 1 precursor and testis expressed sequence 12. The detected amount of these proteins enabled the grouping of asthenozoospermic sperm samples in an unsupervised clustering analysis.
We have identified several proteins present at different amount in asthenozoospermic sperm samples. These proteins could be candidates towards the development of diagnostic markers, and open up the opportunity to gain further insight into the pathogenic mechanisms involved in asthenozoospermia.
弱精子症是不育男性中最常见的表现之一，但在大多数情况下其病因仍不清楚。目前的蛋白质组学工具为鉴定在弱精子症精液样本中差异表达且可能与不育有关的蛋白质提供了机会。
我们使用二维蛋白质组学分析，比较了20份弱精子症样本中101个精子蛋白质点的表达与10份精液供体对照样本的表达。
与对照相比，在弱精子症样本中已鉴定出17个蛋白质点的含量不同。这些蛋白质是细胞骨架肌动蛋白-B、膜联蛋白-A5、细胞色素C氧化酶-6B、组蛋白H2A、催乳素诱导蛋白及其前体、钙结合蛋白-S100A9（2个点）、簇集素前体、二氢硫辛酰胺脱氢酶前体、延胡索酸水合酶前体、热休克蛋白-HSPA2、肌醇-1-单磷酸酶、3-巯基丙酮酸硫转移酶/二烯酰辅酶A异构酶前体、蛋白酶体亚基-PSMB3、精液凝胶蛋白1前体和睾丸表达序列12。这些蛋白质的检测量使得在无监督聚类分析中能够对弱精子症精子样本进行分组。
我们已经鉴定出在弱精子症精子样本中含量不同的几种蛋白质。这些蛋白质可能是诊断标志物开发的候选物，并为进一步深入了解弱精子症的致病机制提供了机会。
