Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Tokyo 153-8505, Japan.
Chemphyschem. 2013 Jun 3;14(8):1618-25. doi: 10.1002/cphc.201300022. Epub 2013 Mar 20.
Direct transport powered by motor proteins can alleviate the challenges presented by miniaturization of microfluidic systems. There have been several recent attempts to build motor-protein-driven transport systems based on simple capturing or transport mechanisms. However, to achieve a multifunctional device for practical applications, a more complex sorting/transport system should be realized. Herein, the proof of concept of a sorting device employing selective capture of distinct target molecules and transport of the sorted molecules to different predefined directions is presented. By combining the bottom-up functionality of biological systems with the top-down handling capabilities of micro-electromechanical systems technology, highly selective molecular recognition and motor-protein-based transport is integrated in a microfluidic channel network.
由马达蛋白驱动的直接运输可以缓解微流控系统小型化带来的挑战。最近已经有一些尝试基于简单的捕获或运输机制来构建马达蛋白驱动的运输系统。然而，为了实现适用于实际应用的多功能设备，应该实现更复杂的分拣/运输系统。在这里，提出了一种采用选择性捕获不同靶分子并将分拣的分子输送到不同预定方向的分拣装置的概念验证。通过将生物系统的自下而上的功能与微机电系统技术的自上而下的处理能力相结合，在微流控通道网络中集成了高度选择性的分子识别和基于马达蛋白的运输。
