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痘病毒端粒的解析：通过保守链交换处理牛痘病毒多联体连接点

Resolution of poxvirus telomeres: processing of vaccinia virus concatemer junctions by conservative strand exchange.

作者信息

Merchlinsky M

机构信息

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.

出版信息

J Virol. 1990 Jul;64(7):3437-46. doi: 10.1128/JVI.64.7.3437-3446.1990.

DOI:10.1128/JVI.64.7.3437-3446.1990
PMID:2352329
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC249602/
Abstract

The replication of vaccinia virus proceeds through concatemeric intermediates which are resolved into unit-length DNA. In vaccinia virus-infected cells, plasmids containing the vaccinia virus DNA junction fragment that connects concatemers are resolved into linear minichromosomes of vector DNA flanked by hairpin loops. Resolution requires two copies of a specific nucleotide sequence conserved among poxviruses and found proximal to the hairpin loop. This study demonstrates that orientation of each sequence with respect to the other as well as to the axis of symmetry is critical for resolution, the processing of plasmids containing heterologous pairs of resolution sites is influenced by mismatched nucleotides between the sites, and the vaccinia virus hairpin in the linear minichromosome is a heteroduplex composed of DNA from each strand of the concatemer junction. A model incorporating site-specific recombination and orientated branch migration is proposed to account for resolution of the vaccinia virus concatemer junction.

摘要

痘苗病毒的复制通过串联中间体进行，这些中间体被切割成单位长度的DNA。在痘苗病毒感染的细胞中，含有连接串联体的痘苗病毒DNA连接片段的质粒被切割成由发夹环侧翼的载体DNA线性微型染色体。切割需要在痘病毒中保守且位于发夹环附近的特定核苷酸序列的两个拷贝。这项研究表明，每个序列相对于另一个序列以及对称轴的方向对于切割至关重要，含有异源切割位点对的质粒的加工受到位点之间错配核苷酸的影响，并且线性微型染色体中的痘苗病毒发夹是由串联体连接的每条链的DNA组成的异源双链体。提出了一个包含位点特异性重组和定向分支迁移的模型来解释痘苗病毒串联体连接的切割。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bc0/249602/a7010621838d/jvirol00062-0306-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bc0/249602/167a61a9775b/jvirol00062-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bc0/249602/733e3122616e/jvirol00062-0305-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bc0/249602/3a6f4efbe542/jvirol00062-0305-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bc0/249602/b4d314b10235/jvirol00062-0306-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bc0/249602/a7010621838d/jvirol00062-0306-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bc0/249602/167a61a9775b/jvirol00062-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bc0/249602/733e3122616e/jvirol00062-0305-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bc0/249602/3a6f4efbe542/jvirol00062-0305-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bc0/249602/b4d314b10235/jvirol00062-0306-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5bc0/249602/a7010621838d/jvirol00062-0306-b.jpg

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A hairpin plastid genome in barley.大麦中的发夹状质体基因组。
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Origin-independent plasmid replication occurs in vaccinia virus cytoplasmic factories and requires all five known poxvirus replication factors.不依赖于起源的质粒复制发生在痘苗病毒的细胞质工厂中，并且需要所有五个已知的痘病毒复制因子。
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Resolution of a Holliday junction by vaccinia topoisomerase requires a spacer DNA segment 3' of the CCCTT/ cleavage sites.痘苗病毒拓扑异构酶对霍利迪连接体的拆分需要在CCCTT/切割位点的3'端有一个间隔DNA片段。
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