Merchlinsky M, Moss B
Cell. 1986 Jun 20;45(6):879-84. doi: 10.1016/0092-8674(86)90562-3.
The junctions, separating unit-length genomes in intracellular concatemeric forms of vaccinia virus DNA, are duplex copies of the hairpin loops that form the ends of mature DNA molecules present in infectious virus particles. Circular E. coli plasmids with palindromic junction fragments were replicated in vaccinia virus-infected cells and resolved into linear minichromosomes with vector DNA in the center and vaccinia virus DNA hairpins at the two ends. Resolution did not occur when the concatemer joint was less than 250 bp or when plasmids were transfected into uninfected cells, indicating requirements for a specific DNA structure and viral trans-acting factors. These studies indicate that concatemers can serve as replicative intermediates and account for the generation of flip-flop sequence variation of the hairpins at the ends of the mature vaccinia virus genome.
痘苗病毒DNA的细胞内串联形式中分隔单位长度基因组的连接点，是形成感染性病毒颗粒中成熟DNA分子末端的发夹环的双链拷贝。带有回文连接片段的环状大肠杆菌质粒在痘苗病毒感染的细胞中复制，并解析为线性微型染色体，其中心为载体DNA，两端为痘苗病毒DNA发夹。当串联接头小于250 bp时或当质粒转染到未感染细胞中时，解析不会发生，这表明对特定DNA结构和病毒反式作用因子有要求。这些研究表明，串联体可作为复制中间体，并解释了成熟痘苗病毒基因组末端发夹的翻转序列变异的产生。
