Merchlinsky M
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
J Virol. 1990 Oct;64(10):5029-35. doi: 10.1128/JVI.64.10.5029-5035.1990.
In replicative forms of vaccinia virus DNA, the unit genomes are connected by palindromic junction fragments that are resolved into mature viral genomes with hairpin termini. Bacterial plasmids containing the junction fragment for vaccinia virus or Shope fibroma virus were converted into linear minichromosomes of vector sequence flanked by poxvirus hairpin loops after transfection into infected cells. Analysis of a series of symmetrical deletion mutations demonstrated that in vaccinia virus the presence of the DNA sequence ATTTAGTGTCTAGAAAAAAA on both sides of the apical segment of the concatemer junction is crucial for resolution. To determine the precise architecture of the resolution site, a series of site-directed mutations within this tract of nucleotides were made and the relative contribution of each nucleotide to the efficaciousness of resolution was determined. The nucleotide sequence necessary for the resolution of the vaccinia virus concatemer junction, (A/T)TTT(A/G)N7-9AAAAAAA, is highly conserved among poxviruses and found proximal to the hairpin loop in the genomes of members of the Leporipoxvirus, Avipoxvirus, and Capripoxvirus genera.
在痘苗病毒DNA的复制形式中，单位基因组通过回文连接片段相连，这些片段会被解析成具有发夹末端的成熟病毒基因组。将含有痘苗病毒或肖普纤维瘤病毒连接片段的细菌质粒转染到受感染细胞后，会转化为由痘病毒发夹环侧翼的载体序列组成的线性微型染色体。对一系列对称缺失突变的分析表明，在痘苗病毒中，串联体连接顶端片段两侧存在DNA序列ATTTAGTGTCTAGAAAAAAA对解析至关重要。为了确定解析位点的精确结构，在这一核苷酸区域内进行了一系列定点突变，并确定了每个核苷酸对解析有效性的相对贡献。痘苗病毒串联体连接解析所需的核苷酸序列(A/T)TTT(A/G)N7 - 9AAAAAAA在痘病毒中高度保守，并且在兔痘病毒属、禽痘病毒属和山羊痘病毒属成员的基因组中位于发夹环附近。
