蛙粪霉属和虫霉属物种的脂肪酶活性。

Lipase activity of Basidiobolus and Conidiobolus species.

作者信息

Okafor J I, Gugnani H C

机构信息

Department of Microbiology, University of Nigeria, Nsukka.

出版信息

Mycoses. 1990 Feb;33(2):81-5. doi: 10.1111/myc.1990.33.2.81.

DOI:10.1111/myc.1990.33.2.81

PMID:2352544

Abstract

Two isolates of Basidiobolus namely, B. haptosporus (ATCC 36600) and B. ranarum (MHB1), and one isolate of Conidiobolus coronatus (C1) were grown in modified Sierra medium for twelve days. Periodic measurements of their lipase activity showed significant activity of the enzyme from the fourth day with a steady increase up to the twelfth day of incubation. The lipases produced by the three isolates were stable over a wide range of pH (4-8) and temperature (30-60 degrees C). Maximum lipase activity for B. haptosporus and C. coronatus was at pH 5.0 and that for B. ranarum was at pH 6.0. The three fungi exhibited a peak of activity at the same temperature, i.e. 35 degrees C.

摘要

两种担子菌分离株，即哈氏担子菌（ATCC 36600）和蛙担子菌（MHB1），以及一种冠状耳霉分离株（C1），在改良的塞拉培养基中培养12天。定期测量它们的脂肪酶活性，结果显示从第四天起该酶就具有显著活性，直至培养的第十二天活性持续稳定增加。这三种分离株产生的脂肪酶在较宽的pH范围（4 - 8）和温度范围（30 - 60摄氏度）内都很稳定。哈氏担子菌和冠状耳霉的脂肪酶活性在pH 5.0时最高，蛙担子菌的脂肪酶活性在pH 6.0时最高。这三种真菌在相同温度即35摄氏度时表现出活性峰值。