Laboratório de Bioquímica de Hemoparasitas e Vetores-LABHEV, Universidade do Estado de Santa Catarina, Avenida Luiz de Camões, no. 2090, Bairro Conta Dinheiro, 88520-000, Lages, SC, Brazil.
Parasitol Res. 2013 Jun;112(6):2361-6. doi: 10.1007/s00436-013-3400-9. Epub 2013 Mar 26.
The aim of this study was to evaluate the changes in hematological and biochemical parameters of blood during acute Trypanosoma evansi infection in Wistar rats. The end points studied were hematologic parameters, red blood cell fragility, iron content, and glutathione and lipid peroxidation levels. Forty-eight animals were infected with trypomastigotes and distributed into five groups according to the level of parasitemia. Twelve non-inoculated animals were used as control. Parasitemia increased progressively, reaching highest scores at 15 days post-inoculation. At this point, several deleterious effects were observed such as an increase in iron content, in osmotic fragility, and in lipid peroxidation index, while glutathione decreased drastically. These changes were highly correlated to parasitemia (p < 0.0001) and among each other (p ≤ 0.001). Hematological indices (Hb, packed cell volume (PCV), red blood cells (RBC), and mean corpuscular hemoglobin concentration) were also correlated to parasitemia (p ≤ 0.0003) but failed to correlate to the other variables. Along with increase in iron, RBC fragility produced a decrease in RBC, PCV, and Hb, but not in mean corpuscular volume. Decrease in glutathione was negatively correlated to the end products of lipid peroxidation, clearly indicating the establishment of a pro-oxidant condition. The results show that the infection causes hematological impairments, increases iron and osmotic fragility, along with marked oxidative stress in red blood cells of rats inoculated with T. evansi.
本研究旨在评估锥虫蓝染色急性伊氏锥虫感染期间血液中血液学和生化参数的变化。研究终点为血液学参数、红细胞脆性、铁含量、谷胱甘肽和脂质过氧化水平。48 只动物感染锥虫后，根据寄生虫血症水平分为五组。12 只未接种的动物作为对照。寄生虫血症逐渐增加，在接种后 15 天达到最高水平。此时，观察到了一些有害影响，如铁含量增加、渗透脆性增加和脂质过氧化指数增加，而谷胱甘肽则急剧下降。这些变化与寄生虫血症高度相关（p<0.0001），且彼此之间也高度相关（p≤0.001）。血液学指标（Hb、红细胞压积、红细胞和平均红细胞血红蛋白浓度）也与寄生虫血症相关（p≤0.0003），但与其他变量无关。随着铁的增加，红细胞脆性导致红细胞、红细胞压积和 Hb 减少，但平均红细胞体积不变。谷胱甘肽的减少与脂质过氧化的终产物呈负相关，这清楚地表明了促氧化剂条件的建立。结果表明，感染导致大鼠血液学损伤，增加铁和渗透脆性，同时伴有明显的红细胞氧化应激。
