Da Silva A S, Pierezan F, Wolkmer P, Costa M M, Oliveiro C B, Tonin A A, Santurio J M, Lopes S T A, Monteiro S G
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
J Comp Pathol. 2010 Feb-Apr;142(2-3):170-6. doi: 10.1016/j.jcpa.2009.10.020. Epub 2009 Dec 1.
Five cats were experimentally inoculated with Trypanosoma evansi in order to evaluate the pathological changes induced by this protozoan infection. Clinical signs observed included vomiting, diarrhoea, hyperthermia, weight loss, facial oedema, corneal opacity, lymphadenopathy and hindlimb instability. Reduction in hematocrit was observed from 7 days post-infection (dpi) (P<0.05). One cat died at 40 dpi and the other four cats were humanely destroyed. Necropsy examination was performed in two cats at 56 dpi and two cats at 120 dpi. Gross findings in all cats included generalized muscle atrophy, pale mucosae, icterus of the subcutaneous and serosal tissue and the intima of arteries, lymphadenopathy and splenomegaly. Other findings included corneal opacity, subcutaneous oedema (mainly of the head) and hydropericardium. Trypomastigotes of T. evansi were observed in impression smears prepared from the aqueous humor. Microscopically, there was lymphoid hyperplasia of the spleen and lymph nodes. The animals with corneal opacity had mild corneal oedema and accumulation of fibrin and inflammatory cells (neutrophils and plasma cells) in the anterior chamber. Similar inflammatory cells infiltrated the iris, ciliary body, corneoscleral limbus and conjunctiva.
为了评估由伊氏锥虫感染引起的病理变化，对五只猫进行了伊氏锥虫的实验性接种。观察到的临床症状包括呕吐、腹泻、体温过高、体重减轻、面部水肿、角膜混浊、淋巴结病和后肢不稳。感染后7天（dpi）观察到血细胞比容降低（P<0.05）。一只猫在40 dpi时死亡，另外四只猫被人道处死。在56 dpi时对两只猫进行了尸检，在120 dpi时对另外两只猫进行了尸检。所有猫的大体检查结果包括全身性肌肉萎缩、黏膜苍白、皮下和浆膜组织以及动脉内膜黄疸、淋巴结病和脾肿大。其他发现包括角膜混浊、皮下水肿（主要在头部）和心包积水。从房水制备的印片中观察到伊氏锥虫的锥鞭毛体。显微镜下，脾脏和淋巴结有淋巴样增生。角膜混浊的动物有轻度角膜水肿，前房有纤维蛋白和炎性细胞（中性粒细胞和浆细胞）积聚。类似的炎性细胞浸润虹膜、睫状体、角膜巩膜缘和结膜。
