Center for Biophotonics Science and Technology, University of California Davis, Sacramento, CA 95817, USA.
Analyst. 2013 May 21;138(10):2906-13. doi: 10.1039/c3an00025g.
Surface-enhanced Raman scattering (SERS) is a promising analytical technique for the detection and characterization of biological molecules and structures. The role of hydrophobic and hydrophilic surfaces in the self-assembly of protein-metallic nanoparticle structures for label-free protein detection is demonstrated. Aggregation is driven by both the hydrophobicity of the surface as well as the charge of the proteins. The best conditions for obtaining a reproducible SERS signal that allows for sensitive, label-free protein detection are provided by the use of hydrophobic surfaces and 16 × 10(11) NPs per mL. A detection limit of approximately 0.5 μg mL(-1) is achieved regardless of the proteins' charge properties and size. The developed method is simple and can be used for reproducible and sensitive detection and characterization of a wide variety of biological molecules and various structures with different sizes and charge status.
表面增强拉曼散射（SERS）是一种很有前途的分析技术，可用于检测和表征生物分子和结构。本文展示了疏水性和亲水性表面在蛋白质-金属纳米粒子结构的自组装中对无标记蛋白质检测的作用。蛋白质的疏水性和所带电荷都促使了聚集。通过使用疏水性表面和每毫升 16×10(11)个纳米粒子，可以获得可重现的 SERS 信号，从而实现灵敏的无标记蛋白质检测，提供最佳条件。无论蛋白质的电荷性质和大小如何，检测限约为 0.5 μg mL(-1)。所开发的方法简单，可用于各种生物分子和具有不同大小和电荷状态的各种结构的重现性和灵敏检测及表征。
