Department of Genetics and Bioengineering, Yeditepe University, Kayışdağı/Kadıköy, Istanbul 34755, Turkey.
Anal Chem. 2010 Sep 15;82(18):7596-602. doi: 10.1021/ac101720s.
Proteins are one of the most versatile groups of molecules with vital functional roles in living systems. Their enormous diversity and structural flexibility make the detection of these molecules a challenging task. A simple and sensitive label-free protein detection method based on assembly of proteins and colloidal silver nanoparticles (AgNPs) on surfaces and surface-enhanced Raman scattering (SERS) is reported. The SERS spectra from the assembled AgNP/protein films show excellent reproducibility and high quality regardless of the proteins' charge status and size. A detection limit down to 0.5 μg/mL for three acidic proteins; BSA, catalase and pepsin, and three basic proteins; cytochrome c, avidin and lysozyme, is easily achieved. The minimum improvement in detection limit is more than 1 order of magnitude compared to the previously reported detection limits using the technique and the approach has the potential for label-free protein detection and identification.
蛋白质是分子中最具多功能性的一类，在生命系统中具有重要的功能作用。它们的巨大多样性和结构灵活性使得这些分子的检测成为一项具有挑战性的任务。本文报道了一种简单、灵敏的基于蛋白质在表面上组装和胶体银纳米粒子（AgNPs）以及表面增强拉曼散射（SERS）的无标记蛋白质检测方法。组装的 AgNP/蛋白质薄膜的 SERS 光谱具有出色的重现性和高质量，无论蛋白质的电荷状态和大小如何。对于三种酸性蛋白质（BSA、过氧化氢酶和胃蛋白酶）和三种碱性蛋白质（细胞色素 c、亲和素和溶菌酶），检测限低至 0.5μg/mL。与先前使用该技术报道的检测限相比，检测限的最小改善超过 1 个数量级，该方法具有用于无标记蛋白质检测和鉴定的潜力。
