College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China.
World J Microbiol Biotechnol. 2013 Aug;29(8):1531-6. doi: 10.1007/s11274-013-1315-3. Epub 2013 Mar 26.
Measuring yeast biomass is important in the processes of microbial fermentations. It has been demonstrated that synchronous light scattering (SLS) signals could be applied for the quantification of model bioparticles such as Saccharomyces cerevisiae. In this study, an improved synchronous light scattering method was developed for yeast biomass estimation. The settlement of yeast cells during SLS signals measuring process was studied, and hydrolysis anionic polyacrylamide was added into yeast suspensions to increase the stability of the cells in liquid environment. By simultaneously scanning both the excitation and emission monochromators of a common spectrofluorometer with same starting excitation and emission wavelength (namely, ∆λ = 0), the SLS intensity was found to be proportional to the yeast concentration in the range from 0 to 4.9 × 10(6) cell/mL (R (2) = 0.9907), the detection limit is 8.1 × 10(3) cell/mL. The developed method exhibited good stability and sensitivity in the recovery test and growth curve drawing process, demonstrating the potential of the method in practical application of biomass estimation.
测量酵母生物量在微生物发酵过程中很重要。已经证明，同步光散射（SLS）信号可用于定量模型生物颗粒，如酿酒酵母。在本研究中，开发了一种改进的同步光散射方法来估计酵母生物量。研究了在 SLS 信号测量过程中酵母细胞的沉降，并向酵母悬浮液中添加水解阴离子聚丙烯酰胺，以提高细胞在液体环境中的稳定性。通过同时以相同的起始激发和发射波长（即 ∆λ = 0）扫描普通荧光分光光度计的激发和发射单色仪，可以发现 SLS 强度与酵母浓度在 0 到 4.9 × 10(6) 细胞/mL 的范围内成正比（R (2) = 0.9907），检测限为 8.1 × 10(3) 细胞/mL。该方法在回收试验和生长曲线绘制过程中表现出良好的稳定性和灵敏度，表明该方法在生物量估计的实际应用中具有潜力。
