Jones H E, Skyring G W
Biochim Biophys Acta. 1975 Jan 23;377(1):52-60. doi: 10.1016/0005-2744(75)90285-5.
The type and the amount of end products resulting from sulfite reduction catalysed by a single partially purified desulfoviridin preparation from Desulfovibrio gigas were shown to depend upon the enzymic assay conditions employed. Both manometric and spectrophotometric assays were used, with reduced methyl viologen serving as the electron donor in each system. Trithionate, thiosulfate, tetrathionate and sulfide were identified as possible end products. In the manometric assays, sulfide production was favoured by high reduced methyl viologen concentrations, low sulfite concentrations and a pH value of 7.0 as opposed to 6.0. In the spectrophotometric assays, results approaching the stoichiometric conversion of sulfite to sulfide were obtained only at high initial reduced methyl viologen concentrations.
由巨大脱硫弧菌的单一部分纯化的脱硫绿素制剂催化亚硫酸盐还原产生的终产物类型和数量，取决于所采用的酶分析条件。使用了测压法和分光光度法分析，在每个系统中，还原型甲基紫精作为电子供体。连三硫酸盐、硫代硫酸盐、连四硫酸盐和硫化物被鉴定为可能的终产物。在测压法分析中，高浓度还原型甲基紫精、低亚硫酸盐浓度和pH值7.0（相对于6.0）有利于硫化物的产生。在分光光度法分析中，只有在高初始还原型甲基紫精浓度下才能获得接近亚硫酸盐化学计量转化为硫化物的结果。
